丝氨酸／苏氨酸激酶15基因的过表达与胃癌细胞增殖有关  

The association of serine/threonine kinase 15 gene overexpression with the proliferation of gastric cancer cells

作　　者：兰斌[1] 陈雪华[1] 刘炳亚[1] 瞿颖[1] 蔡劬[1] 戴起宝[2] 张健[3] 陈绍勤[2] 朱正纲[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院外科 上海消化外科研究所,200025 [2]福建医科大学附属第一医院肿瘤外科 [3]福建医科大学附属协和医院神经内科

出　　处：《中华消化杂志》2006年第4期250-253,共4页Chinese Journal of Digestion

基　　金：国家973重点基础研究发展规划基金(2002CB713700)

摘　　要：目的探讨胃癌细胞及组织中丝氨酸／苏氨酸激酶15(STK15)mRNA和蛋白质表达与临床病理指标、胃癌细胞增殖的关系。方法采用实时定量PCR及Western blot分别检测正常胃黏膜上皮细胞株GES-1、胃癌细胞株SUN-16、KATOIII、MKN45、AGS、N87及60份新鲜切除的胃癌组织中 STK15基因mRNA及蛋白质表达。RNA干扰技术(RNAi)抑制MKN45、AGS、N87细胞株STK15表达,流式细胞仪检测细胞周期变化,MTT法检测细胞增殖速率变化,数据行秩和检验及t检验。结果 SUN-16、KATOⅢ、MKN45、AGS、N87中STK15基因mRNA及蛋白质表达均明显高于GES-1;60份胃癌组织中STK15 mRNA及蛋白质表达水平中位值分别为3．175×10-3与1．261,明显高于正常组织的0．532×10-3与0．224(P<0．05);胃癌组织中STK15 mRNA水平与胃癌分化及浸润深度有关(P< 0．05);而蛋白质表达水平与胃癌分化有关(P<0．05)。抑制STK15表达后,MKN45、AGS、N87细胞株 G2期DNA含量细胞分别为(26．1±6．1)％、(25．6±7．9)％及(20．6±6．1)％,明显高于对照组的(12．5 ±4．9)％、(10．9±4．4)％及(8．7±3．5)％(P<0．05);细胞增殖速率减慢(P<0．05)。结论 STK15 的过表达可能在胃癌发生发展中起促进作用,可作为胃癌某些生物学行为新的判定指标;STK15的过表达与胃癌细胞增殖有关。Objective To elucidate the association of serine/threonine kinase 15 （STK15） mRNA and protein expressions with the clinicopathological parameters of gastric carcinoma and gastric cancer cell proliferation. Methods Expression of STK15 mRNA and protein were measured by real-time PCR and Western blot respectively in normal gastric epithelial cell line GES-1, gastric cancer cell lines SUN- 16, KATOm ,MKN45, AGS, N87 and 60 cases of fresh gastric carcinoma tissues, STK15 expression in MKN45,AGS, N87 cell lines was inhibited by RNA interference, The distribution of cell cycle was detected by flow cytometry. The proliferation of cells was evaluated by MTT assay. Data were analyzed by Sum rank and t test. Results STK15 mRNA and protein levels in gastric cancer cells were substantially higher than that in normal gastric ephithelial cells （P〈0.05）, The medians of STK15 mRNA and protein expression levels in 60 cases of gastric cancer tissues were 3. 175× 10^-3 and 1. 261, significantly higher than 0. 532× 10^-3 and 0. 224 in normal tissues（P〈0. 05）. STK15 mRNA overexpression level in gastric carcinoma tissues was closely related to tumor differentiation and invasion （P〈0. 05）, and STK15 protein level to tumor differentiation （P〈0. 05）, After inhibition of STK15 expression, the percentage of G2 cells in MKN45, AGS, N87 cell lines were （26.1 ± 6.1）%, （25.6± 7.9）%, （20.6 ±6. 1）%, significantly higher than （12. 5±4. 9） %,（10. 9±4.4） %,（8. 7±3.5）% in control groups （P 〈0.05）, meanwhile, cells also proliferated slowly （P〈0. 05）, Conclusions Overexpression of STK15 may promote gastic carcinogenesis and development of gastric cancer, and can be used as a novel marker for determining biological behaviours of gastric cancer. Overexpression of STK15 is related to the proliferation of gastric cancer cells.

关 键 词：胃肿瘤 丝氨酸/苏氨酸激酶15 RNA干扰 增殖 

分 类 号：R735.2[医药卫生—肿瘤]