斑纹鲟凝集素(CFL)的初步研究 Ⅱ.CFL的分子组成及性质鉴定  

The preliminary studies of a lectin(CFL) from Charybdis feriatus Ⅱ.The molecular composition and the characterization of CFL

作　　者：戴聪杰[1] 王桂忠[1] 李少菁[1] 艾春香[1] 

机构地区：[1]厦门大学海洋学系

出　　处：《海洋学报》2006年第3期98-102,共5页

基　　金：福建省自然科学基金资助项目(B0410027);泉州市科学技术局重点科技计划资助项目(Z200239);国家863项目计划资助项目(2002AA603013)

摘　　要：采用生化方法对斑纹鲟（Charybdis feriatus）凝集素进行了初步研究．结果表明，斑纹鲟的血清中有动物红细胞和微生物细胞凝集活性的凝集素（Charybdis feriatus lectin，CFL），其活力表现依赖于Ca^2＋，并为多种糖所抑制；CFL对40℃以上的温度敏感，其活力在pH6．0～8．0内稳定．经巯基乙醇处理和未经巯基乙醇处理的CFL在SDS-PAGE上均为一条蛋白带，说明斑纹鲟凝集素不含二硫键，且只有一种亚基，分子量为40．8kDa，CFL氨基酸组成分析表明其富含酸性氨基酸。Lectin of Charybdis feriatus （shortly call CFL）was studied by biochemistry methods. The results show that the lectin agglutinates every kinds RBC from different animals and microbial cells tested widely in the experiment, as a Ca^2＋ dependent lectin CFL is inhibited by various sugars. CFL is sensitive to the temperature over 40℃ and to the pH over 9.0 or below 5.0. No matter adding or not adding -S-S- reductant to the sample of CFL, it demonstrated one single band on plate SDS-PAGE. It shows that CFL does not contain -S-S- , and its molecular mass is 40.8kDa. The analysis result of amino acid composition of CFL indicates that acidity amino acids were abundant.

关 键 词：斑纹鲟凝集素(CFL) 糖抑制 热稳定性 分子量 氨基酸组成 

分 类 号：Q959.233[生物学—动物学]