磁性纳米颗粒Fe_3O_4固定化纤维素酶的光谱学研究  

Spectroscopy Study of the Immobilized Cellulose of Magnetic Nanoparticles Fe_3O_4

作　　者：王玫[1] 宋芳[1] 汪世龙[2] 吴庆生[1] 

机构地区：[1]同济大学化学系,上海200092 [2]同济大学生命科学与技术学院,上海200092

出　　处：《光谱学与光谱分析》2006年第5期895-898,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(50572074)资助项目

摘　　要：以氨水作沉淀剂,用共沉淀法制备了磁性纳米颗粒Fe3O4,并以此为载体,通过碳化二亚胺的活化作用将纤维素酶固定化,通过傅里叶红外和重复多次催化实验证实纤维素酶在磁性纳米颗粒上的固定,透射电镜表征了固定化酶微粒的形貌.用DNS分光光度法测定固定化纤维素酶的活性,研究表明磁性固定化酶的催化作用的最适温度为60℃和pH值为3.94～5.50.结果表明,磁性固定化纤维素酶具有比自由酶更好的热稳定性,贮存稳定性和更广泛的pH值适用范围,为纤维素的转化和利用效率的提高提供了理论基础.Fe3O4 magnetic nanoparticles were synthesized by chemical coprecipitation method using ammonia as the precipitator in the present paper. And then cellulase was immobilized on Fe3O4 magnetic nanoparticles via carbodiimide activation, which was testified by FTIR and lots of repeating catalysis experiments. The morphology of nanoparticles immobilized by cellulase was characterized by TEM, and the activity of cellulase was measured by DNS spectrophotometry. The optimum temperature（60℃） and pH value （3.94-5.50） for the catalysis ablility of immobilized cellulose were studied. The result showed that compared with the native enzyme the cellulase immobilized on Fe3O4 magnetic nanoparticles has the advantages of thermal stability, storage stability, and more extensive optimum pH value.

关 键 词：磁性纳米颗粒Fe3O4 固定化 纤维素酶 光谱学 

分 类 号：O657[理学—分析化学]