鬼臼酰肼镍(Ⅱ)金属配合物与DNA的相互作用  

Studies on the Interaction of the Metal Complex of Hydrazide of Podophyllic Acid with DNA

作　　者：王平红[1] 张岐[1] 王流芳[2] 宋玉民[3] 曲建强[2] 刘营前[2] 

机构地区：[1]海南大学精细化工海南省重点实验室,海南海口570228 [2]兰州大学应用有机国家重点实验室,甘肃兰州730000 [3]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《光谱学与光谱分析》2006年第5期941-943,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20361003);海南省自然科学基金(20302)资助项目

摘　　要：利用吸收光谱、荧光光谱、DNA热变性研究了鬼臼酰肼金属配合物与小牛胸腺DNA之间的相互作用。在金属配合物的存在下,DNA的紫外吸收光谱产生了明显的减色效应。实验结果表明,鬼臼酰肼金属配合物与DNA之间主要以嵌入方式相结合。The interaction between the metal complex of hydrazide of podophyllic acid and calf thymus （CT） DNA was studied by using absorption spectra, fluorescence spectra and DNA heat denaturation. It was found that the intensity of the maximal absorption peaks from absorption spectra is weakened in the presence of the metal complex of hydrazide of podophyllic acid compared with that in the absence of the metal complex. All the experimental results show that the intercalation mode was proved to exist between HDPP-Ni complexes and CT DNA.

关 键 词：金属配合物 溴化乙锭 小牛胸腺DNA 

分 类 号：R914.3[医药卫生—药物化学] Q523[医药卫生—药学]