用大孔树脂从大豆乳清中纯化大豆异黄酮  

Purification of soybean isoflavones from soybean whey with macroporous resin

作　　者：井乐刚[1] 张永忠[2] 

机构地区：[1]哈尔滨师范大学阿城学院生命科学系,黑龙江阿城150301 [2]东北农业大学理学院应用化学系,黑龙江哈尔滨150030

出　　处：《化工学报》2006年第5期1209-1213,共5页CIESC Journal

基　　金：国家"十五"重大科技攻关计划(2001BA501A02B);黑龙江省十五科技攻关项目(GA01B40408);黑龙江省教育厅科学技术研究项目(10541285).~~

摘　　要：In order to purify isoflavones by column chromatography from soybean whey,the discarded by-product in the production of soybean protein isolate,the adsorption and desorption characteristics of four kinds of macroporous resins for isoflavones were comparatively investigated.D4006 resin was chosen as the adsorbent.Before column chromatography, the soybean whey was ultrafiltrated and extracted with ethyl acetate to remove protein and other components.The sample containing isoflavones was passed through a column(30 mm×800 mm) packed with D4006 resin at the flow rate of 1 ml·min -1 at 25℃.The concentration of isoflavones in the sample was 1 mg·ml -1.The volume of the sample was 2.67 liters per liter resin.After adsorption, the resin was gradient-eluted by using aqueous ethanol solution (from 20% to 95% ethanol, by volume) at the flow rate of 1 ml·min -1 at 75℃.The eluate was vacuum-distilled to remove ethanol and water, and then was vacuum-dried to obtain a solid product.Under these conditions, a product with 72.5% of soybean isoflavones determined by HPLC was obtained.In order to purify isoflavones by column chromatography from soybean whey, the discarded byproduct in the production of soybean protein isolate, the adsorption and desorption characteristics of four kinds of macroporous resins for isoflavones were comparatively investigated. D4006 resin was chosen as the adsorbent. Before column chromatography, the soybean whey was ultrafiltrated and extracted with ethyl acetate to remove protein and other components. The sample containing isoflavones was passed through a column （430 mm×800 mm） packed with 04006 resin at the flow rate of 1 ml ·min^-1 at 25℃. The concentration of isoflavones in the sample was 1 mg·ml^-1. The volume of the sample was 2.67 liters per liter resin. After adsorption, the resin was gradient-eluted by using aqueous ethanol solution （from 20% to 95% ethanol, by volume） at the flow rate of 1 ml· min^-1 at 75℃. The eluate was vacuumdistilled to remove ethanol and water, and then was vacuum-dried to obtain a solid product. Under these conditions, a product with 72.5 % of soybean isoflavones determined by HPLC was obtained.

关 键 词：大豆异黄酮 大豆乳清 大孔树脂 纯化 

分 类 号：TQ028.15[化学工程]