小鼠肝癌微波消融对宿主Th2/Th1免疫应答的影响  

Microwave Ablation of Mouse Hepatocellular Carcinoma:Effects on the Th2/Th1 Immune Response in the Host

作　　者：刘大全[1] 冯炼强[2] 吕明德[1] 谭进富[1] 王竹[1] 周忠信[1] 

机构地区：[1]中山大学附属第一医院肝胆外科,广州510080 [2]中山大学基础医学院免疫教研室,广州510080

出　　处：《热带医学杂志》2006年第5期490-492,共3页Journal of Tropical Medicine

基　　金：国家自然科学基金(No.30471686)

摘　　要：目的探讨小鼠肝癌微波消融(microwaveablation,MA)对宿主Th2/Th1免疫应答的影响。方法建立C57BL/6J小鼠Hepa1-6细胞移植肝癌模型,对肿瘤微波消融或手术切除,取正常、荷瘤、手术后及MA后四组小鼠,每组6只,用ELISA法检测各组周围血血浆Th1(IL-12I、FN-γ)和Th2(IL-4I、L-10)细胞因子水平(pg/ml)。结果荷瘤组比正常组,细胞因子IL-10增加,IL-4I、L-12和IFN-γ下降(均P>0.05)。MA组较荷瘤组,IL-12、IFN-γ显著上升(均P<0.01),IL-4明显降低(P<0.05),IL-10有所下降(P>0.05)。手术组比荷瘤组,IL-12I、FN-γ上升,IL-4I、L-10下降(均P>0.05)。MA组比手术组,IL-12明显上升(P<0.05),IFN-γ升高,IL-4和IL-10下降(均P>0.05)。结论MA原位灭活小鼠肝癌可促进宿主免疫应答的Th2/Th1偏移,并且MA较手术切除具有较强的免疫刺激效应。Objective To investigate the effect of microwave ablation （MA） of hepatoceilular carcinoma （HCC） on the Th2/Th1 immune response in tumor-bearing mice. Methods A HCC model was established by subcutaneous inoculation of syngeneic Hepal-6 ceils into C57BL/6J mice. The tumors were treated with MA or by resection. Four groups of animals （6 mice per group）, namely normal, tumor-hearing, resection-treated and MA-treated, were set up. Concentrations of Th1 （IL-12, IFN-γ） and Th2 （IL-4, IL-10） cytokines in plasma were determined by ELISA. Results Compared with the normal mice, the levels of IL-4, IL-12 and IFN-γ in tumour-bearing mice were decreased while the level of IL-10 was increased （P 〉0.05）. For the comparisons between MA and tumor-bearing group, only the levels of IL-12 and IFN-γ in the MA group were markedly higher than the tumour-bearing mice （P〈0.01）. Compared with tumorhearing mice, the levels of IL-12 and IFN-γ in mice treated by resection were increased, but the levels of IL-4 and IL- 10 were lowered （P〉0.05）. The level of IL-12 in MA group was obviously higher than the mice with tumour resection （P〈0.05）. With the exception of IL-10 and IL-4, the level of IFN-γ in mice treated with MA was higher than the mice with tumour resection （P〉0.05） . Conclusion A skewing of Th2/Th1 immune response was seen in mice after MA of HCC. MA of the tumor may cause a stronger immune reaction than resection.

关 键 词：肝癌 微波消融 细胞因子 Th2/Th1偏移 

分 类 号：R735.7[医药卫生—肿瘤]