温郁金对人胃癌裸鼠移植瘤生长和环氧合酶-2表达的影响  

Effects of Common Turmeric on the Growth of Transplanted Tumor and Expression of Cyclooxygenase-2 in Nude Mice Implanted with Human Gastric Cancer Cells

作　　者：王佳林[1] 吕宾[1] 倪桂宝[1] 麻林爱[1] 徐毅[1] 

机构地区：[1]浙江省中医院消化科

出　　处：《胃肠病学》2006年第5期277-280,共4页Chinese Journal of Gastroenterology

基　　金：浙江省中医药管理局资助(No.2003C037)

摘　　要：温郁金为常用传统中药材,研究发现其组分具有抗癌作用。目的:研究温郁金对人胃癌裸鼠移植瘤生长和环氧合酶-2(COX-2)表达的影响,探讨其抑制胃癌的作用机制。方法:将人胃癌细胞系SGC-7901种植于裸鼠皮下,建立胃癌移植瘤模型。待瘤体长至约2mm时,12只荷瘤裸鼠随机分为治疗组和对照组,分别予温郁金提取液0.3ml和等量0.9%NaCl溶液灌胃,每日2次,连续7周。每周测量一次瘤体大小,7周后处死裸鼠,测定肿瘤重量,以免疫组化ElivisionTMplus法检测肿瘤组织COX-2的表达。结果:治疗组裸鼠移植瘤体积和重量均显著低于对照组,抑瘤率为42.5%;肿瘤组织COX-2阳性染色强度和阳性细胞百分率亦显著低于对照组。结论:温郁金对人胃癌裸鼠移植瘤的生长具有明显抑制作用,抑制肿瘤组织中COX-2的表达可能是其作用机制。Background： Common turmeric is a frequently used crude conventional Chinese medicine. Is was found that its ingredients had anti-tumor effect. Aims： To investigate the effects of common turmeric on the growth of transplanted tumor and expression of cyclooxygenase-2 （COX-2） in nude mice implanted with human gastric cancer cells, and to study the mechanism of its antieancer effect. Methods： Human gastric cancer cell line SGC-7901 was implanted subcutaneously to induce transplanted tumor in nude mice. When the diameter of transplanted tumor grew up to 2 mm, 12 experimental mice were randomly divided into two groups： in the treatment group the nude mouse was given 0.3 ml common turmeric extracts intragastrically twice a day for 7 weeks, in the control group the nude mouse received saline of the same volume, simultaneously. The size of the tumors was measured weekly. After 7 weeks, the mice were killed and the transplanted tumors were weighed and immunohistochemical method （Elivision^TM plus kit） was used to detect the expression of COX-2 in tumorous tissues. Results： The volume and weight of the transplanted tumors in the treatment group were less than those in the controls, the inhibition rate of the tumor weight was 42.5%; so were the staining intensity and area of COX-2 expression in tumorous tissues. Conclusions： Common turmeric has a significant inhibitory effect on the growth of transplanted tumors in nude mice, which might be related to the downregulation of COX-2 expression.

关 键 词：郁金 胃肿瘤 环氧合酶2 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤] R285.5[医药卫生—临床医学]