维生素A阻断沥青烟气诱发小鼠皮肤肿瘤的实验研究  

The Experimental Study of the Inhibitory Effect of Vitamin A on the Mouse Skin Neoplasm Induced by Pitch Smoke

作　　者：王江[1] 郝志芳[1] 李新荣[1] 魏丽[1] 赵庆夏[1] 

机构地区：[1]河南医科大学病理学教研室

出　　处：《河南肿瘤学杂志》1996年第2期85-87,共3页Henan Journal of Oncology

基　　金：河南省教委自然基金

摘　　要：将１５０只昆明种小鼠随机分为三组（Ⅰ、Ⅱ、Ⅲ组）。Ⅰ、Ⅱ组小鼠每天同时在一染毒柜中暴露于煤焦沥青烟气３小时，柜内沥青烟气浓度平均为０．１７ｍｇ／Ｌ。Ⅱ组小鼠每周再接受维生素Ａ灌胃两次，每次给维生素Ａ醋酸酯７５０ＩＵ。烟气暴露共４个月，另再观察４个月，灌胃持续至小鼠处死。Ⅲ组为空白对照组。８个月中，Ⅰ、Ⅱ组小鼠皮肤肿瘤的总发生率分别为８３．３％和５９．２％，Ⅱ组明显低于Ⅰ组（Ｐ＜０．０５）。Ⅰ、Ⅱ组皮肤鳞癌的发生率分别为６６．７％和３０．６％；其中Ⅰ组有８例癌发生了转移，而Ⅱ组仅有１例。Ⅱ组皮肤癌的发生率和转移率均显著低于Ⅰ组（Ｐ＜０．０１）。此外，Ⅱ组发瘤小鼠的平均荷瘤数也明显少于Ⅰ组（Ｐ＜０．０５）。Ⅲ组无皮肤肿瘤发生。结果提示，维生素Ａ可有效地阻断由沥青烟气所致的皮肤肿瘤的发生、癌变和转移，或可用于高危险职业工人皮肤癌的预防。In this study,150 Kunming mice were randomly divided into three groups,(group Ⅰ,ⅡandⅢ ).The mice in group Ⅰ and Ⅱ were exposed to coal tar pitch smoke(CTPS) togather in a sealed box 3 hours daily in an average concentration of 0.17mg/m3.The mice in group Ⅱreceived catheter feeding of vitamin A acetic ester(750 IU in 0.2 ml peanut oil each time)twice a week.The exposure to CTPS lasted for 4 months.Another 4-month observation was taken after that.Catheter feeding lasted untill the death of the mouse.Group Ⅲ was designed as a control.In the 8 months,the total incidences of the skin neoplasms in group Ⅰ and Ⅱwere 83.3% and 59.2%. The incidence in group Ⅱ was markedly lower than that in group Ⅰ(P<0.05).The incidences of skin cancers in group Ⅰ and Ⅱ were 66.7% and 30.6% respectively,of which there were 8 cases of metastasis in group Ⅰ and 1 in group Ⅱ. Both incidence and metastatic rate of skin cancer in group Ⅱ were significantly lower than those in group Ⅰ(P<0.01).In addition,the mice in group Ⅱ had much fewer tumors than groupⅠ(P<0.05). The results suggest that vitamin A could effectively inhibite the tumorigenesis,canceration and metastasis of the skin neoplasms induced by pitch smoke.

关 键 词：维生素A 皮肤肿瘤 沥青烟气 小鼠 

分 类 号：R739.5[医药卫生—肿瘤]