尾加压素Ⅱ对人脐静脉内皮细胞凋亡的影响  

Effect of urotensin Ⅱon apoptosis of human umbilical vein endothelial cells

作　　者：顾晓龙[1] 严全能[1] 张园[1] 李志梁[1] 刘映峰[1] 张宏兵[2] 冼江[2] 

机构地区：[1]南方医科大学附属珠江医院心内科,广州市研究生510282 [2]广州军区广州总医院医学实验科,510010

出　　处：《实用医学杂志》2006年第11期1228-1230,共3页The Journal of Practical Medicine

基　　金：国家重大发展基础研究项目973子课题(编号G200056905)

摘　　要：目的:研究尾加压素Ⅱ(UⅡ)对人脐静脉内皮细胞(HUVEC)的诱导凋亡作用,以及这种诱导作用与细胞内游离Ca2+的关系。方法:(1)光镜观察培养的静脉内皮细胞形态。(2)分成浓度组和时间组:浓度组加入不同浓度的UⅡ(10-9～10-6mol/L),并将硫氮唑酮加入对照组及10-7mol/L组;时间组则观察10-7mol/LUⅡ作用后不同时间段HUVEC的凋亡率。(3)流式细胞仪分析细胞周期,计算凋亡率。(4)电镜检测细胞凋亡。结果:(1)UⅡ促进HUVEC凋亡,二者呈时间依赖性(P<0.05);二者呈明显量效关系(P<0.05)。(2)硫氮唑酮可抑制UⅡ的诱导作用。结论:UⅡ可诱导HUVEC凋亡,其机制可能与[Ca2+]i升高有关。Objective To study the effect of urotensin Ⅱ （UⅡ） on inducing apoptosis of human umbilical vein endothelial cells （HUVEC） and the relationship between [Ca^2＋]i and this induction of apoptosis. Methods （1）The cultured HUVEC morphology was identified by light microscopy. （2）Group A：U Ⅱ with various concentrations （10^-9 10^-6 mol/L） was added into the cultured HUVEC, respectively. In addition, dihiazem of 400 ng/mL was added into the control group and the U Ⅱwith 10^-7 mol/L concentration group; Group B：after the U Ⅱwith 10^-7 mol/L concentration was added, the apoptotic rate was observed in different time periods （0 h, 12 h, 24 h,48 h, and 72 h）. （3）The cell cycle was detected by flow cytometry and the apoptotic rate was counted. （4）The apoptosis was examined by electron microscopy. Results （1） U Ⅱinduced the typical apoptosis of HUVEC in a time-dependent manner （P〈0.05） and a characteristic dosedependent manner （ P〈0.05）; （2） Dihiazem might significantly suppress the U H-induced apoptosis. Conclusion U Ⅱ could induce the apoptosis of HUVEC, which may be associated with the increase of [Ca^2＋]i.

关 键 词：内皮细胞 血管升压素类 血液动力学过程 细胞凋亡 

分 类 号：R363[医药卫生—病理学]