Fluorescence Determination of Artemisinin Using Hemoglobin as Catalyst and Pyronine B as Substrate  

作　　者：CHEN Lihua ZHANG Yongkang YIN Hong LIU Liuzha YANG Zhaoxia SHEN Hanxi 

机构地区：[1]College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, Hunan, China [2]College of Chemistry, Nankai University, Tianjin, 300071,China

出　　处：《Wuhan University Journal of Natural Sciences》2006年第3期704-708,共5页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Natural Science Foundation of China(20075012) ;the Scientific Research Foundation of Hunan Educational Commit-tee (02C313)

摘　　要：Fluorescence decrease ratio was applied to determine of arte misinin （qinghaosu, QHS） based on the catalytic effect of hemoglobin （Hb） using tetraethyldiaminoxanthenyl chloride （Pryonine B, PB） as monitor in this work, Experimental results show that the catalytic characteristics of Hb for QHS, being expressed as Michaelis-Menten parameters, Km, Vmax and Kcat are 2.8×10^-5 mol·L^-1, 4.2×10^-5 mol·L^-1·s^-1 and 280 s^-1 , respectively. Like hemin, enzymatic bioactivities of Hb is inhibited by both deactivated agents and high temperature whereas enhanced by ethanol. With the catalytic action of Hb, quantitative method of QHS can be established based on the fluorescence decrease of PB and linear relationship between the fluorescence decrease ratio F0/F and the concentration of QHS is in the range of （0.0-1.1）×10^-6 mol·L^-1 with detection limit （3σ） being 7. 2×10^-9 mol·L^-1, The concentration of QHS in the media of plasma or urine was detected using this method.Fluorescence decrease ratio was applied to determine of arte misinin （qinghaosu, QHS） based on the catalytic effect of hemoglobin （Hb） using tetraethyldiaminoxanthenyl chloride （Pryonine B, PB） as monitor in this work, Experimental results show that the catalytic characteristics of Hb for QHS, being expressed as Michaelis-Menten parameters, Km, Vmax and Kcat are 2.8×10^-5 mol·L^-1, 4.2×10^-5 mol·L^-1·s^-1 and 280 s^-1 , respectively. Like hemin, enzymatic bioactivities of Hb is inhibited by both deactivated agents and high temperature whereas enhanced by ethanol. With the catalytic action of Hb, quantitative method of QHS can be established based on the fluorescence decrease of PB and linear relationship between the fluorescence decrease ratio F0/F and the concentration of QHS is in the range of （0.0-1.1）×10^-6 mol·L^-1 with detection limit （3σ） being 7. 2×10^-9 mol·L^-1, The concentration of QHS in the media of plasma or urine was detected using this method.

关 键 词：HEMOGLOBIN ARTEMISININ pyronine B fluorescence decrease ratio 

分 类 号：O657.39[理学—分析化学]