2株猪圆环病毒ORF2基因的克隆测序  

Cloning and Sequence Analysis of ORF2 Gene of Two PCV-2 Strains

作　　者：张晋川[1] 

机构地区：[1]江苏畜牧兽医职业技术学院

出　　处：《动物医学进展》2006年第6期81-83,共3页Progress In Veterinary Medicine

摘　　要：对分离到的2株猪圆环病毒PCV-2型的ORF2基因进行了扩增,将获得的PCR产物分别与T载体连接,获得了2个毒株的ORF2基因阳性克隆。序列分析表明,这2个分离株与其他中国分离株同源性高达99%以上,同处于一个基因簇,但和中国BF分离株同源性却只有92.3%。而荷兰分离的NL-PMWS-2株与中国分离株的同源性却在99%以上,表明中国流行的PCV-2和国外分离株有密切关系。The ORF2 genes from 2 strains of PCV-2 were amplified and determined by PCR technique. The nucleotide sequence similarities among China strains were above 99% except one strain（BF）,the similarities of BF and other China strains were below 93%, the similarities of the NL-PMWS-2 and other China strains also were above 99%.

关 键 词：猪圆环病毒2型 ORF2基因 克隆 序列分析 

分 类 号：S852.659.2[农业科学—基础兽医学] S858.28[农业科学—兽医学]