Avastin阻断VEGF促横纹肌肉瘤细胞株RH4增殖的作用  

Blocking Enhancive Effect of Vascular Endothelial Growth Factor on Proliferation of Rhabdomyosarcoma Cell Line RH4 by Avastin

作　　者：李志刚[1] 史艳侠[2] 彭柔君[2] 朱美琴[2] 万一元[2] 

机构地区：[1]中山大学基础医学院,广东广州510080 [2]华南肿瘤学国家重点实验室

出　　处：《癌症》2006年第6期718-722,共5页Chinese Journal of Cancer

摘　　要：背景与目的:血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)及其通路的研究是目前研究的热点之一,但其在横纹肌肉瘤中的研究鲜有报道,其作用机制尚不完全清楚。本研究通过检测横纹肌肉瘤细胞株RH4中VEGF及其受体的表达情况,以了解VEGF在横纹肌肉瘤中的作用及机制,并研究Avastin阻断VEGF促细胞增殖的作用。方法:使用RT-PCR方法检测RH4细胞株中VEGF、VEGFR1、2、3mRNA的表达;使用ELISA方法检测培养液上清中VEGF的分泌水平;使用Westernblot方法检测VEGFR1蛋白的表达水平;使用直接计数的方法检测VEGF、Avastin对RH4细胞增殖的影响。结果:RT-PCR和ELISA结果显示在RH4细胞中存在VEGF的表达和分泌;在RH4细胞中仅有VEGFR1表达,VEGFR2和VEGFR3均为阴性;Westernblot的结果进一步证实了VEGFR1在RH4中的表达。VEGF可直接促进RH4细胞的增殖,在0～100ng/ml的区间其促进增殖的作用随着浓度的增加而增加。10～40μg/mlAvastin可以阻断VEGF的促增殖作用。结论:在横纹肌肉瘤细胞RH4中VEGF可以通过自分泌的方式作用于自身,并通过VEGFR1促进肿瘤细胞的增殖,Avastin可以阻断VEGF的促增殖作用。BACKGROUND ＆ OBJECTIVE： Vascular endothelial growth factor （VEGF） signal pathway is a hot spot in recent tumor research, but its role in rhabdomyosarcoma （RMS） has rarely been reported, and its mechanism is unclear. This study was to investigate the expression of VEGF and its receptors （VEGFR） in RMS cell line RH4 to reveal the mechanism of VEGF signal pathway, and explore the therapeutic value of Avastin in RMS in vitro. METHODS： Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression of VEGF, VEGFR1, VEGFR2, and VEGFR3 mRNA in RH4 cells. ELISA was used to detect the concentration of VEGF in supernatant. Western blot was used to detect the expression of VEGFR1 protein. The effects of VEGF and Avastin on RH4 celt growth were evaluated by direct cell counting. RESULTS： RH4 cells expressed VEGF at a high level and secreted VEGF to culture media. RH4 cells only expressed VEGFR1, did not express VEGFR2 and VEGFR3. VEGF promoted the growth of RH4 cells in a dose-dependent manner within a concentration range of 0-100 ng/ml; this effect could be blocked by 10-40μg/ml Avastin. CONCLUSION. VEGF can promote the growth of RMS cell through VEGFR1, and this effect can be blocked by Avastin.

关 键 词：横纹肌肉瘤 RH4细胞株 血管内皮细胞生长因子 贝伐单抗 

分 类 号：R738.5[医药卫生—肿瘤]