人胶质瘤相关基因表达变化的基因芯片分析  

Gene microarray analysis of genes on changes of human glioblastoma gene expression

作　　者：程迎新[1] 唐文渊[1] 

机构地区：[1]重庆医科大学附属第一医院神经外科,重庆400016

出　　处：《现代肿瘤医学》2006年第6期659-664,共6页Journal of Modern Oncology

摘　　要：目的:运用基因芯片技术建立恶性胶质瘤和其对应的癌旁组织的基因表达图谱,分析其差异表达基因,从基因表达水平上揭示胶质瘤的生物学行为。方法:采用上海生物工程有限公司提供的含13824个基因位点的人类基因表达谱芯片(SBC-R-HC-100-20)对WHOIII级和WHOIV级的胶质瘤和相应的瘤周组织进行检测。结果:筛选出包括与DNA结合、转录和转录因子、蛋白质翻译、细胞周期、转移等相关的表达差异的基因共275个,其中143个表达上调,132个表达下调。结论:恶性胶质瘤的发生过程中存在许多基因表达的改变。基因芯片技术应用于恶性胶质瘤演化相关基因研究,可以方便快速地缩小研究范围,有效地筛选出目的基因进行深入研究。Objective：To study the different gene expression of glioblastomas and the tissue around the glioblastomas by gene microarray analysis in order to reveal the biological behaviour of glioblastoma in the level of gene. Methads; The human gene microarray chip （SBC - R - HC - 100 -20） manufacted by the Biotechnology Limited Company of Shanghai have 13824 gene situs and was adopt to detected the glioblastoma in the level of WHOⅢ and WHOIV and the tissue of the glioblastomas. Results： Between the glioblastomas and the tissue around the glioblastomas , there were 275 genes differential about DNA - binding,transcription and transcription factor, proteo - translation,ceU cycle, metachoresis, in which 143 genes up -regulated, 132 genes down -regulated. Conclusion：There are many changes about gene expression in the development of the glioblastoma. Application of the cDNA microarray in the research of glioblastoma can conveniently and quickly deflate the study circumscription, effectually select objective - gene to research deeply.

关 键 词：基因芯片 胶质瘤 差异表达 复发 

分 类 号：R730.264[医药卫生—肿瘤]