宫内缺氧对子代大鼠脂肪肝发病的影响  

Influence of intrauterine hypoxia on pathogenesis of fatty liver in offspring rats

作　　者：苏瑞娟[1] 吕国荣[1] 王振华[1] 李伯义[1] 刘彦英[1] 何韶铮[1] 金鹏[1] 

机构地区：[1]福建医科大学附属第二医院超声科

出　　处：《世界华人消化杂志》2006年第11期1048-1051,共4页World Chinese Journal of Digestology

基　　金：福建省自然科学基金资助课题;No.C0540002

摘　　要：目的:探讨宫内缺氧及出生后联合高脂饮食和追加缺氧对子代大鼠脂肪肝发病的影响．方法:建立大鼠宫内缺氧模型,并对子代大鼠施加高脂饮食和追加缺氧干预,测量子代大鼠肝脏超声灰阶值,处死后取肝脏组织,行常规 HE染色,用CMIAS多功能真彩色病理图像分析系统行半自动定量分析肝脏脂肪变性细胞数目．结果:宫内缺氧、高脂饮食和追加缺氧的主效应(在脂肪变性细胞数F值分别为:13．348, 45．45,18．541;均P<0．05)在脂肪肝发病中均有促进作用;在脂肪肝的发病中,宫内缺氧与高脂饮食、高脂饮食与追加缺氧间的交互作用 (在脂肪变性细胞数F值分别为:5．723,5．933; 均P<0．05)存在;超声灰阶均值与脂肪变性细胞数间呈正相关(r=0．810,P<0．05)．结论:宫内缺氧可增加子代大鼠对高脂饮食所致的脂肪肝的敏感性．AIM： To study the roles of simple fetal hypoxia, or combined with high-fat diet and the second hypoxia, in adiposis hepatica of offspring rats. METHODS： Rat intrauterine hypoxia models were established, and then their offspring were exposed to high-fat diet or/and second hypoxia. The histogram of hepatic ultrasound was measured by B-ultrasound examination. Pathological observation was performed by HE staining for calculating the number of fatty degenerative hepatic cells in the offspring rat with CMIAS system. RESULTS： The number of the fatty degenerative cells in liver tissues was significantly different between the intrauterine hypoxia group and controls, the high-fat diet group and controls, as well as between the second hypoxia group and controls （F = 13.348, 45.45, 18.541, respectively; all P 〈 0.05）. Moreover, there was an interactive effect between intrauterine hypoxia and high-fat diet, or intrauterine hypoxia and high-fat diet （F values for the number of the fatty degenerative cells were 5.723 and 5.933, respectively, both P 〈 0.05）. In addition, a positive correlation （r = 0.810, P 〈 0.05） was observed between the number of fatty degenerative cells in the offspring liver tissues and the B-ultrasound gray scale value. CONCLUSION： Intrauterine hypoxia may increase the risk of fatty liver in the offspring of rats exposed to long-term high-fat diet.

关 键 词：宫内缺氧 脂肪肝 超声直方图 大鼠 

分 类 号：R575.5[医药卫生—消化系统]