ASC在大鼠胰腺炎肺损伤发病中的作用及中药清胰汤对其表达的影响  

Role of apoptosis-associated speck-like protein containing a Caspase recruitment domain in pathogenesis of lung injury induced by severe acute pancreatitis in rats and effect of Qingyitang on its expression

作　　者：任双义[1] 陈海龙[2] 

机构地区：[1]大连医科大学附属第二医院普外科,辽宁省大连市116023 [2]大连医科大学附属第一医院普外科,辽宁省大连市116011

出　　处：《世界华人消化杂志》2006年第11期1058-1063,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30271667

摘　　要：目的:探讨ASC(apoptosis-associated specklike protein containing a Caspase recruitment domain)在胰腺炎肺损伤发病机制中的作用及中药清胰汤对胰腺炎肺损伤大鼠ASC及其相关的细胞因子表达的影响．方法:SD大鼠40只,随机分4组:假手术组、胰腺炎肺损伤组、清胰汤治疗组、善宁治疗组．采用15 g／L去氧胆酸钠逆行注入胰胆管诱发胰腺炎肺损伤模型．通过自动生化分析仪检测大鼠血清淀粉酶;采用ELISA法测定血清 IL-1β水平;RT-PCR检测肺组织ASC mRNA的表达;免疫组织化学法检测肺组织和胰腺组织ASC蛋白的表达．通过检测肺组织髓过氧化物酶(MPO)活性、肺湿／干质量比值和肺组织病理切片判定肺损伤程度．结果:胰腺炎肺损伤组血清淀粉酶和IL-1β水平较假手术组显著升高(77 632±5934 nkat／L vs 16 303±1450 nkat／L,P<0．01和386．26± 50．54 ng／L vs99．11±18．43 ng／L,P<0．01)．清胰汤治疗组血清淀粉酶(17 420±1867 nkat／L)和 IL-1β(105．23±20．21 ng／L)水平较肺损伤组血清淀粉酶(77 632±5934 nkat／L,P<0．01)和IL- 1β水平(386．26±50．54 ng／L,P<0．01)明显下降．善宁治疗组血清淀粉酶(20 437±123 nkat／L) 和IL-1β水平(109．63±19．98 ng／L)较肺损伤组血清淀粉酶(77 632±5934 nkat/L,P<0．01)和 IL-1β水平(386．26±50．54 ng／L,P<0．01)明显下降．假手术组肺组织内ASC mRNA表达较弱．胰腺炎肺损伤组肺组织及胰腺组织内ASC蛋白表达较假手术组显著上调,其灰度值有显著性差异(24．86±5．23 vs 54．12±7．91,P<0．01和 25．46±4．21 vs 52．32±8．10,P<0．01),清胰汤治疗组两者表达显著下调(48．97±7．45vs24．86 ±5．23,P<0．01和49．48±8．13vs25．46±4．21, P<0．01)．善宁治疗组两者的表达亦显著下调 (48．69±5．87 vs 24．86±5．23．P<0．01和49．11± 6．41vs25．46±4．21,P<0．01)．清胰汤治疗组和善宁治疗组肺组织MPO活性减低(5．33±0．50 nkat／L vs 18．67±AIM： To study the pathogenesis of ASC （apoptosis-associated speck-like protein containing a Caspase recruitment domain） in acute lung injury （ALI） induced by severe acute pancreatitis （SAP） and the effect of Chinese medicine Qingyitang on the expression of ASC and cytokines in rats. METHODS： Forty Sprague Dawley （SD） rats were randomized into four groups： sham operation group （SHAM, n = 10）, ALI models （ALI, n = 10）, ALI＋ Sandostatin （SS, n = 10）, ALI ＋ Qingyitang （QYT, n = 10）. ALI during SAP was induced by retrograde infusion of 15 g/L sodium deoxycholate into the bili-pancreatic duct in SD rats. All the rats were killed 24 hours after operation and treatment. Serum amylase was measured by an automated HITACHI analyzer. The level of myeloperoxidase （MPO） in pulmonary tissues was measured by enzyme chemistry assay. Serum interleukin-1β （IL -1β） protein was measured by enzyme-linked immunosorbent assay （ELISA）. The expression of ASC mRNA and protein in pulmonary and pancreatic tissues were detected by reverse transcription polymerase chain reaction （RT- PCR） and immunohistochemistry, respectively. The severity of pulmonary injuries was evaluated by MPO, the ratio of wet to dry lung tissues and pathological changes of rat pulmonary tissues. RESULTS： The serum amylase and IL-1β protein levels were increased significantly in group ALI in comparison with those in SHAM group, respectively （77 632 ± 5934 nkat/L vs 16 303 ± 14,50 nkat/L and 386.26 ± 50.54 ng/L vs 99.11 ± 18.48 ng/L, both P 〈 0.01）, but they were decreased significantly in QYT group （17 420 ± 1867 nkat/ L vs 77 632 ± 5934 nkat/L, 105.23 ± 20.21 ng/L vs 386.26 ± 50.54 ng/L, both P 〈 0.01） and group SS （20 437 ± 123 nkat/L vs 77 632 ± 5934 nkat/L and 109.63 ± 19.98 ng/L vs 386.26 ± 50.54 ng/L, both P 〈 0.01） as compared with those in ALI group.The expression of ASC mRNA and protein were highly up-regulated in ALI group as compared with those in SHAM group （24.8

关 键 词：重症急性胰腺炎 肺损伤 ASC 白细胞介素1 

分 类 号：R576[医药卫生—消化系统]