重症急性胰腺炎大鼠单核细胞趋化蛋白1的表达  

The expression of monocyte chemotactic protein-1 in severe acute pancreatitis rats

作　　者：周国雄[1] 徐岷[1] 张弘[1] 黄介飞[1] 魏群[1] 江枫[1] 肖明兵[1] 

机构地区：[1]南通大学附属医院消化病科,226001

出　　处：《江苏医药》2006年第6期555-557,共3页Jiangsu Medical Journal

摘　　要：目的探讨单核细胞趋化蛋白1(MCP-1)在重症急性胰腺炎(SAP)发病及其相关的急性肺损害(ALI)中的作用。方法48只雄性SD大鼠随机分为SAP组和手术对照组,采用向胆胰管逆行注射5%牛磺胆酸钠建立大鼠SAP模型,术后1、3、6和12 h分别观察血清淀粉酶,胰腺和肺组织病理组织学改变,酶联免疫固相吸附试验(ELISA)法测定血清中MCP-1水平,免疫组织化学法和逆转录-聚合酶链反应(RT-PCR)法检测胰腺和肺组织中MCP-1蛋白和MCP-1 mRNA的表达。结果SAP组术后61、2 h血清中MCP-1水平高于手术对照组(P<0.05),胰腺组织和肺组织中的MCP-1蛋白和MCP-1 mRNA的表达亦高于手术对照组(P<0.05或P<0.01),且MCP-1的表达与胰腺和肺组织的病理学改变呈正相关。结论MCP-1可能在SAP发病及其相关的ALI中起了重要的作用。Objective To investigate the expression of monocyte chemotactic protein-1 （MCP-1） in severe acute pancreatitis（SAP） rats and its role in acute lung injury（ALI）. Methods Forty-eight SD rats were randomly divided into SAP group and operative control group. The SAP model was induced by retrograde infusion of 5% sodium taurocholate into bili-pancreatic duct. The severity of SAP was assessed by serum anylase level and the histological changes of the pancreas and lung. Serum level of MCP-1 was detected by enzyme-linked immunoadsordent assay（ELISA） at 1,3,6 and 12 hours after operation. Expression of MCP-1 proteins and MCP-1 mRNA in the pancreas and lung were detected by immunohistochemistry and reverse transcriptase polymerase chain reaction（RT-PCR）, respectively. Results Serum levels of MCP-1 at 6 or 12 hours after operation in SAP group were significantly higher than those in operative control group（P〈0.05）. At the same time, the expressions of MCP-1 and MCP-1 mRNA in the pancreas and lung at 6 or 12 hours after operation in SAP group were significantly higher than those in operative control group（P〈0, 05 or P〈0. 01 ）. Expression of MCP-1 was correlated with the histological changes of the pancreas and lung. Conclusion MCP-1 may play an important role in the pathogenesis of SAP and ALI.

关 键 词：胰腺炎 单核细胞趋化蛋白1 急性肺损害 

分 类 号：R739.41[医药卫生—肿瘤] R657.51[医药卫生—临床医学]