健康成人刺激性全唾液流速及一氧化氮含量的增龄性变化  

Age-related changes of stimulated whole saliva flow rate and nitric oxide level in healthy adults

作　　者：徐俊峰[1] 刘丽[2] 

机构地区：[1]浙江省立同德医院口腔科,310012 [2]浙江大学附属口腔医院修复科

出　　处：《实用口腔医学杂志》2006年第3期317-320,共4页Journal of Practical Stomatology

摘　　要：目的：研究不同年龄阶段健康成人的刺激性全唾液流速及唾液一氧化氮含量的变化。方法：将97例健康成人分成4个年龄组，分别为青龄组（20～39岁）、中龄组（40～59岁）、老龄组（60～79岁）、长寿组（80岁以上）。在上午8：00～10：00空腹，以2．5ml／L洗必太漱口，以20ml／L柠檬酸刺激舌背前1／3，以双蒸水漱口后采集全唾液5min，记录样本容量，并计算其流速。用硝酸还原酶法检测唾液中NO浓度，并计算其唾液中NO的单位时间含量。结果：（1）青龄组刺激性全唾液流速高于中龄组、老龄组、长寿组（P〈0．05）；中龄组、老龄组、长寿组之间唾液流速无显著性差异（P〉0．05）；（2）不同年龄组间两两比较，刺激性全唾液NO浓度无显著性差异（P〉0．05）；青龄组NO单位时间含量显著高于中龄组、老龄组和长寿组（P〈0．05），中龄组、老龄组、长寿组之间的NO单位时间含量无显著性差异（P〉0．05）。结论：（1）中老年人群的刺激性全唾液流速显著低于青年人群；（2）随年龄增长，健康成人刺激性全唾液中的NO浓度未发生明显变化，但其单位时间含量呈降低趋势。Objective：To study age-related changes of stimulated whole saliva flow rate and nitric oxide level in healthy adults. Methods ：97 healthy adults were divided into four age groups：20 -39 years （youth group）, 40 -59 years （middle-aged group）, 60-79 years （elderly group）, and 80 years and over （nmcrobian group）. The volunteers were asked to restrain from breakfast in the morning. After mouth rinse with 2.5 ml/L chlorhexidine ,20 ml/L citrate was dropped on the anterior third of the tongue dorsum of the subjeets for two minutes at the pace of 1 drop every 20 seconds. Then the subjects rinsed their mouth 3 times with double-distilled water within 1 minute, then saliva was collected for 5 minutes. The volume of saliva was documented. Samples were frozen at - 70℃ until assayed. The rates were calculated. Nitric oxide concentrations were measured with nitrate reductase and the Griess reagent, and then nitric oxide output （mol/min） was calculated. Results： （1） The flow rate of stimulated whole saliva in youth group was significantly higher than that in middle-aged, aged and macrobian groups（ P〈0.05 ）. Flow rates among the latter three groups were not significantly different（ P〉0.05 ）. （2） There were no significant differences in nitric oxide concentrations between any two of the groups. Output of nitric oxide in youth group was significantly higher than that in middle-aged, aged and macrobian groups （ P〈0.05 ）. Outputs of nitric oxide among the latter three groups were not significantly different（ P〉0.05 ）. Conclusion：Nitric oxide exists in saliva of healthy adults. Stimulated whole saliva flow rate and nitric oxide output decreases with aging.

关 键 词：唾液 流速 一氧化氯 增龄性变化 

分 类 号：R780.2[医药卫生—口腔医学]