人成牙本质细胞L型钙离子通道α_1亚基D亚型特异性基因的克隆  

Cloning of L type calcium channel α_1 D subunit specific gene of human odontoblasts

作　　者：吕海鹏[1] 史俊南[1] 赵守亮[1] 张伟[2] 滑玮[3] Athony J.Smith 

机构地区：[1]第四军医大学口腔医学院,710032 [2]第四军医大学基础部微生物学教研室 [3]第四军医大学西京医院 [4]英国伯明翰大学牙科学院

出　　处：《实用口腔医学杂志》2006年第3期347-349,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金资助项目(资助号:30471889)

摘　　要：目的:克隆人成牙本质细胞L型钙离子通道α1亚基D亚型的特异性基因。方法:采用RT-PCR方法,从人成牙本质样细胞系中克隆人成牙本质细胞L型钙离子通道α1亚基的特异性基因片段,将其亚克隆入pUC18载体进行序列测定。结果:从人成牙本质样细胞系中获得900bp的特异性片段。序列分析表明,与Gen-Bank登陆的人L型钙离子通道α1亚基D亚型的特异性基因序列完全一致。结论:成功地从人成牙本质样细胞系中克隆到人成牙本质细胞L型钙离子通道α1亚基D亚型的特异性基因序列。Objective：To clone L type calcium channel α1 D subunit specific gene of human odontoblasts. Methods： L type calcium channel α1 D subunit specific gene of human odontoblasts was cloned from human odontoblast-like cell line by RT-PCR, target fragment was inserted into pUC18 vector and sequenced. Results：A 900 bp fragment was obtained from human odontoblast-like cell line. The sequence analysis showed that it was 99.9% homologous to human L type calcium channel α1 D subunit specific gene reported by Genebank. Conclusion： The specific sequence of L type calcium channel α1 D subunit of human odontoblast is successfully cloned from human odontoblast-like cell line.

关 键 词：人成牙本质细胞 L型钙离子通道α1 亚基D亚型 人成牙本质样细胞系 基因克隆 

分 类 号：R780.2[医药卫生—口腔医学]