Slit蛋白在地鼠颊囊癌变过程中的意义及与血管形成的关系  

Slit expression and its correlation with microvascular dense of hamster buccal-pouch carcinogenesis

作　　者：赵媛[1] 王丽京[2] 韩冰[1] 马宇光[3] 张洁[1] 

机构地区：[1]兰州大学口腔医学院基础教研室,730000 [2]广东药学院基础学院病理教研室 [3]兰州大学口腔医学院

出　　处：《实用口腔医学杂志》2006年第3期361-364,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金资助项目(30470858)

摘　　要：目的：探讨Slit蛋白在地鼠颊囊癌发展过程中的表达变化及与肿瘤新生血管之问的关系。方法：采用6～8周龄叙利亚金黄地鼠60只，建立DMBA诱发的颊囊癌模型。免疫组化法检测在颊囊癌癌变过程中Slit蛋白、血管内皮生长因子（VEGF）、范德华因子（v-WF）的动态表达，计数新生血管微血管密度（microvascular dense，MVD）的变化。结果：Slit蛋白在原位癌和鳞癌中高表达，与癌前病变和正常黏膜相比有显著性差异（P〈0．05），VEGF阳性表达率及微血管密度（MVD）与Slit蛋白的表达呈一致性。结论：Slit蛋白在地鼠颊囊癌的发展中起重要作用，其机制可能是通过促进肿瘤新生血管的形成而发挥促癌作用的。Objective：. To observe slit expression on the model of DMBA induced cheek pouch caricnoma in hamsters in various stages, so as to elucidate the relationship between slit expression and angiogenesis. Methods： Carcinogenesis model （HBPCa） was established in 60 hamsters by topical application of 5 g,/L DMBA in left buccal pouch three times per week for 14 weeks. Slit expression, vascular endothelial growth factor （VEGF） and von Willebrand factor （v-WF） were examined by immunohistochemical staining, microvascular dense（ MVD ） was measured 6 - 16 weeks respectively after application of DMBA. Results：Carcinoma was developed in all the hamsters. Slit, VEGF and v-WF expression and MVD were all increased with the development of carcinoma（ P〈0.05 or P〈0.01 ）. Slit expression was positively correlated with MVD（ P =0.01 ）. Conclusion：Slit expression is correlated with MVD and may be a novel factor in carcinogenesis.

关 键 词：SLIT 颊囊癌 金黄地鼠 血管形成 

分 类 号：R739.8[医药卫生—肿瘤]