喉癌中STK15基因扩增及表达的研究  

The investigation of STK15 gene amplification and overexpression in laryngeal squamous cell carcinoma

作　　者：叶燕[1] 李福才[1] 王淑云[1] 李婵媛[1] 袁海明[1] 赵旭[1] 富伟能[1] 徐振明 孙开来[1] 

机构地区：[1]中国医科大学基础医学院医学遗传学教研室,沈阳110001 [2]空军463医院耳鼻喉科

出　　处：《中华医学遗传学杂志》2006年第3期326-329,共4页Chinese Journal of Medical Genetics

基　　金：辽宁省自然科学基金项目(2001101039);国家863项目(2002BA711A0818)~~

摘　　要：目的研究喉癌中STK15基因扩增、mRNA和蛋白表达增高在喉癌发生、发展中的作用。方法应用差异PCR方法检测40例喉鳞状细胞癌及癌旁正常对照组织STK15基因扩增的情况;应用逆转录PCR方法检测同批标本STK15mRNA表达情况;用免疫组织化学方法分析STK15蛋白表达。结果肿瘤组织中STK15基因扩增率为35%(14/40);STK15mRNA表达增高占67.5%(27/40);STK15蛋白表达的阳性率为72.5%(29/40);将扩增与表达的结果与喉癌患者的临床各项指标进行统计学分析,STK15基因扩增、STK15mRNA表达增高与肿瘤的分化程度显著相关(P<0.05);STK15蛋白表达与肿瘤的分化程度和病理分级显著相关(P<0.05)。结论喉癌中STK15基因扩增、mRNA和蛋白表达水平增高,导致中心体复制异常、染色体不稳定,可能在喉癌的发生及恶性进展中起一定作用。Objective To investigate the role of SIK15 gene amplification and overexpression to genesis and development of laryngeal squamous cell carcinoma （LSCC）. Methods STK15 gene ampliiieation in 40 eases carcinoma tissues and normal tissues as control was detected by differential PCR approach. STK15 mRNA and protein levels were evaluated by reverse transcription-polymerase chain reaction（RT-PCR） and immunohistochemistry method, Results In 40 LSCC eases, SIK15 gene amplification was found in 14 tumor tissues（35% ）, mRNA overexpression in 27 tumor tissues （67.5%）, and protein upregulated in 29 tumor tissues（72.5% ）. Statistics analysis showed that STK15 gene amplification and mRNA overexpression were obviously associated to differentiation degree of LSCC, and protein overexpression was closely associated with both differentiation degree and pathological grades of LSCC. Conclusion This research resuits suggest that S1K15 gene amplification contributes to its mRNA and protein overexpression through affecting the exact replication of centrosome and separation of chromosomes. SIK15 gene thus plays a role in LSCC oneogenosis and malignant progression.

关 键 词：喉鳞状细胞癌 STK15基因 差异-聚合酶链反应 基因扩增 

分 类 号：R739.65[医药卫生—肿瘤]