潮汕地区汉族人群12个Y短串联重复序列基因座遗传多态性分析  

An analysis for genetic polymorphism of twelve Y short tandem repeat loci in Chaoshan populations

作　　者：刘静文[1] 胡盛平[1] 李飚[1] 

机构地区：[1]广东省汕头大学医学院分子生物学中心,515031

出　　处：《中华医学遗传学杂志》2006年第3期352-354,共3页Chinese Journal of Medical Genetics

基　　金：广东省自然科学基金(000819);国家教育部留学回国人员科研启动基金(外教司[2001]345号);汕头大学研究与发展基金(L00007);汕头市重点科技计划项目(汕府科[2005]116号)~~

摘　　要：目的调查潮汕地区汉族人群12个Y染色体短串联重复序列基因座的遗传多态性。方法应用PowerPlexTMY荧光标记复合扩增系统,对潮汕地区121名无关男性个体血样进行12个Y染色体短串联重复序列基因座的复合扩增,用ABIPRISM3100遗传分析仪对扩增产物进行检测分析。结果DYS19、DYS437、DYS389I、DYS389II、DYS438、DYS439、DYS393、DYS391、DYS390、DYS392基因座检出4～7个基因型,DYS385检出35个等位基因组,各基因座遗传多样性(genediversity,GD值)分布在0.4445～0.9525之间,DYS385基因座最高。结论上述12个Y染色体短串联重复序列基因座构成的单倍型在潮汕人群中具有较高的遗传多态性,适用于法医个体识别和亲权鉴定、遗传学及人类学的相关研究。Objective To study the genetic polymorphism of twelve short tandem repeat loci of Y chromosome in Chaeshan Han populations. Methods Twelve Y short tandem repeat loci, of which the template DNAs were extracted from blood samples of 121 unrelated Chaoshan Han individuals, were amplified by using the PowerPlexTM Y system. The PCR products were genotyped with ABI PRISM 3100 genetic analyzer, Results From the loci DYS19, DYS437, DYS389Ⅰ,DYS389Ⅱ,DYS438, DYS439, DYS393, DYS391, DYS390, and DYS392, 4-7 alleles were checked out; from the loci DYS385, 35 alleles were observed. The overall observed haplotype diversity reached 0.9976. DYS391 was the locus with the lowest gene diversity （0.4445）, while DYS385 had the highest gene diversity （0.9525）. The 12 Y-STR loci are highly polymorphic and suitable to forensic casework, population genetics and anthropology studies.

关 键 词：Y染色体 短串联重复序列 遗传多态性 单倍型 

分 类 号：R394[医药卫生—医学遗传学]