无血清专用培养基体外快速培养临床级树突状细胞的实验探讨  

Rapid In Vitro Generation of Clinical Grade Dendritic Cells in Serum-free Medium

作　　者：周清[1] 吴一龙[2] 郭爱林[2] 王坤[2] 徐崇锐[1] 杨学宁[2] 

机构地区：[1]中山大学附属第三医院胸外科 [2]广东省人民医院肿瘤中心,广州市510630

出　　处：《中国肿瘤临床》2006年第11期605-607,613,共4页Chinese Journal of Clinical Oncology

基　　金：卫生部科学研究基金(编号:98-2-377);广东省科技厅重点基金资助(编号:99M04903G;2KM04402S)

摘　　要：目的:探讨采用无血清专用培养基从肺癌患者外周血单核细胞快速培养树突状细胞(DCs)的方法。方法:将10例肺癌患者外周血单核细胞同时采用含人AB型血清培养基和无血清DCs专用培养基(DCMedium)培养,5例于第7天加入促成熟细胞因子组合,第9～10天收获成熟DCs,另5例于第5天加入促成熟细胞因子组合,且TNF-α剂量加大5倍,第7天收获成熟DCs。结果:DCMedium培养的DCs形态变化快,细胞出现突起的时间早;9～10天收获的两种培养基培养的DCs性能无差异;7天收获的DCs中,DCMedium培养的DCs得率和纯度、共刺激分子表达水平、刺激T细胞增殖能力均优于含血清培养基。结论:无血清DCs专用培养基可以在体外快速培养临床级DCs,为肿瘤免疫治疗奠定基础。Objective： To explore rapid generation of mature dendritic cells from peripheral blood mononuclear cells （PBMC） in serum-free medium. Methods： The PBMC of 10 patients with lung cancer were cultured in DC medium containing 5% AB human serum and in serum-free DC medium. Cytokines were added on the fifth day or the seventh day to promote maturation, and the 7-day cultures showed increased levels of TNF-α. Results： PBMC changed to DCs rapidly in DC medium. In the 9 to 10-day cultures, there was no difference between the two media. In the 7-day cultures, DCs cultured in DC medium alone had higher yield, higher purity, higher levels of costimulatory molecules, and higher activity than those cultured in DC medium with AB human serum. Conclusion： Clinical grade DCs can be cultured rapidly in serum-free DC medium and can be used in tumor immunotherapy.

关 键 词：树突状细胞 无血清培养基 免疫治疗 

分 类 号：R734.2[医药卫生—肿瘤]