中青年脑梗死患者血管紧张素原基因M235T多态性的研究  

The study of angiotensinogen gene M235T polymorphism with cerebral infarction in young and middle-aged adults.

作　　者：琚小红[1] 牛小媛[1] 王利军[1] 李雷利[1] 武艳琴[1] 赵嘉惠[2] 张华屏[2] 

机构地区：[1]山西医科大学第一临床医院神经内科,山西太原030001 [2]山西医科大学实验中心分子生物学实验室

出　　处：《中国优生与遗传杂志》2006年第6期13-15,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨血管紧张素原(AGT)基因M235T多态性与中青年脑梗死(C I)的关系。方法应用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)对62例脑梗死患者,58例正常人进行AGT基因M235T多态性分析。结果脑梗死组AGT基因T235等位基因频率为79.84%,235TT基因型频率为64.52%,对照组分别为73.28%和55.17%。两者的差异无统计学意义χ2=1.09,P=0.296;χ2=1.443,P=0.230,235MT、235MM基因型频率两组相比也无差异(P>0.05)。结论AGT基因M235T多态性可能与中国山西汉族中青年人群C I发病无关。Objective： To explore the relationship between angiotensinogen （AGT） gene M235T polymorphism and cerebral infarction in young and middle - aged adults. Methods ： M235T polymorphism of AGT gene from peripheral blood leukocytes was examined in 62 cases with CI and 58 noninfarct controls by polymerase chain reaction （PCR） amplification and restriction fragment length polymorphism （RFLP）. Results ： The frequencies of AGT gene T235 allele and 235TT genetype were 79. 84% and 64. 52% respectively which are little higher than those of the control group （73.28% and 55.17% respectively）. There is no statistical difference be- tween them （χ^2= 1.09, P = 0. 296 ; χ^2 = 1. 443, P = 0, 230 ）. Conclusions ： There might be no association between AGT gene M235T and cerebral infarction in young and middle -aged adults.

关 键 词：血管紧张素原 多态性 脑梗死 限制性片段长度多态性分析法 

分 类 号：R743.33[医药卫生—神经病学与精神病学]