小鼠髓系树突状细胞4-1BB及4-1BBL表达调节  

The expression regulation of the costimulatory molecules 4-1BB and 4-1BBL on the bone marrow-derived dendritic cells

作　　者：张烽[1] 马倩茹[1] 席泓[1] 周桓[1] 张学光[1] 顾宗江[1] 

机构地区：[1]苏州大学医学生物技术研究所免疫学教研室,苏州215007

出　　处：《现代免疫学》2006年第3期183-186,共4页Current Immunology

基　　金：国家自然科学基金资助项目(30271205);江苏省临床免疫学重点实验室基金资助项目

摘　　要：探讨小鼠髓系树突状细胞(bone marrow-derived dendritic cells,BMDC)共刺激分子4-1BB及其配体4-1BBL表达的变化。将促DC成熟活化因子CD40L-CHO、TNF-α、LPS和IFN-γ,免疫负性调节因子IL-10以及各成熟活化因子与IL-10联合加入BMDC中,观察BMDC上4-1BB及4-1BBL表达的变化。结果显示,加入成熟活化因子的各组BMDC上4-1BB及4-1BBL表达与对照组相比有显著上调(P<0.05)。而IL-10组与对照组相比两者的表达显著下调(P<0.05),且各成熟活化因子与IL-10联合应用与单用IL-10组相比,BMDC上4-1BB和4-1BBL表达上调,有显著性差异(P<0.05)。提示成熟活化因子不仅能上调BMDC上4-1BB和4-1BBL的表达并且能有效拮抗mIL-10对BMDC上4-1BB和4-1BBL表达的下调作用。This study was to investigate the expression changes of the costimulatory molecules 4-1BB and its ligand 4-1BBI. on the mouse bone marrow-derived dendritic cells （BMDC）, in which BMDC were cultured with DC mature and activating factors such as CD40L, TNF-α, LPS and IFN-γ as well as negative immuno-regulatory factor IL-10, and then expression changes of 4-1BB and 4-1BBL on BMDC were investigated. The results showed that the expressions of 4-1BB and 4-1BBL on BMDC cul tured with DC mature and activating factors were obviously up-regulated in comparison with control group （P〈0.05）. In con trast, their expressions on BMDC cultured with IL-10 were obviously down regulated toward control group （P〈0.05）. DC mature and aetivating factors in the culture of BMDC with IL-10 could reverse its down regulatory effect compared with IL-10 group （P〈0.05）. These findings implieate that DC mature and activating factors may not only up-regulate, but also resist the down-regulatory effect of IL-10 with the expressions of 4-1BB and 4-1BBL on BMDC.

关 键 词：树突状细胞 4-1BB 4-1BBL 

分 类 号：R392.1[医药卫生—免疫学]