唇受精的细胞学研究  

CYTOLOGICAL STUDY ON FERTILIZATION IN OF HEMIBARBUS LABEO

作　　者：甘光明[1] 张耀光[2] 张贤芳[2] 王志坚[2] 

机构地区：[1]东南大学遗传中心,南京210009 [2]西南师范大学生命科学学院,重庆400715

出　　处：《水生生物学报》2006年第3期284-291,共8页Acta Hydrobiologica Sinica

基　　金：重庆市科委攻关项目(No.2001-6630);三峡库区生态环境教育部重点实验室基金(No.03121);东南大学基础医学院青年科学基金(No.2004A106)资助

摘　　要：唇精孔器属深凹陷、短孔径型。精子在受精后2s到达精孔管、5s进入卵子。受精后8—15min,卵子进入第二次减数分裂后期。受精后10min,开始形成雄性原核。受精后20min,进入第二次减数分裂末期。受精后25min,雌性原核形成。受精后30—35min,雌性原核向雄性原核移动。受精后40min,雌雄原核接近。受精后50min,雌雄原核结合。受精后70min,受精卵进入第一次有丝分裂中期,受精后80min,进入第一次有丝分裂后期,受精后120min,进入末期。卵黄降解与其内部或外周小泡的泡状缺口紧密相关。雌雄原核结合是精子星光扩张、牵引和细胞质流动的共同结果。有多精入卵的现象。The fertilized eggs of Hemibarbus labeo have been observed by TEM and OM, and mostly events of fertilization cytology have been studied, too. The result suggest： The micropylar apparatus comprising of vestibule and micropylar tube is deeply sunken with a short tunnel, the diameter of the micropylar tube is about 2. 143 μm, and the diameter of the sperm is about 1.445 μm. Before sperm penetrates, the chromosomes are at metaphase in the ovum, following prophase and preceding anaphase, during which the chromosomes are aligned along the metaphase plate. About 2s after fertilization in 17℃, sperms arrive at micropylar tube; 3s, a sperm is penetrating into micropylar tube, 5s, sperms enter into cytoplasm of ovum where the cytoplasm is sunken; 30s, some yolk granules begin to decompose; 35s, the spermaster comes into being; 5min, we find the phenomena of polyspermy in a few of the ovum, which is rare in teleost; but the sperms can＇t come into being spermaster; 8--15min, the chromosome are at anaphase, and 20min, at telophase of meiosis, later, the second polar body expels, and the blastoderm forms in animal hemisphere; 25min, both the male pronucleus and the female pronucleus can be seen; 30--35min, the female pronucleus move towards the male pronucleus, at the same time, both the pronucleuses move to the pivot of blastoderm; 40min, both the pronucleuses press close, and 50min, conjugate into zygote, and then, the zygotic nucleus expands; 70min, chromosomes are situated at metaphase of the first karyokinesis, 80min, at anaphase, and 120min, at telophase, the oosperm is segmentalized in daughter cells. 140min, chromosomes of the daughter cells are situated at metaphase of the second karyokinesis, while the daughter cells don＇t separate. There have two hypothesizes to explain the mechanism how the male pronucleus and the female pronucleus conjugate. One is the Cytoplasm Flowage, which thinks the cytoplasm＇ flowage in ovum result in the conjugation. The other is the Spermaster Traction, which thinks the conju

关 键 词：唇(鱼骨) 受精细胞学 雌雄原核结合 受精膜外举 

分 类 号：Q24[生物学—细胞生物学]