Effects of Glucagon-Like Peptide 1 on PDX-1, PAX-6 and NKx2.2 Gene Expressions in Isolated Pancreatic Islets  被引量:1

Effects of Glucagon-Like Peptide 1 on PDX-1, PAX-6 and NKx2.2 Gene Expressions in Isolated Pancreatic Islets

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作  者:Dai Cui Wei Tang Cuiping Liu Kuanfeng Xu Chao Liu 

机构地区:[1]Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

出  处:《Journal of Nanjing Medical University》2006年第3期141-144,共4页南京医科大学学报(英文版)

基  金:135 Project Foundation of Jiangsu Province (NO2001)

摘  要:Objective: To observe the effect of glucagon-like peptide 1 (GLP-1) on the gene expressions of transcription factors (PDX-1, PAX-6 and NKx2.2 ) in freshly isolated rat pancreatic islets and investigate the associated physiological and therapeutic implication of GLP-1. Methods: The isolated rat islets were incubated with 10 nmol/L GLP-1 for 1, 3 and 5 days, respectively. Total cellular RNA was extracted and the expressions of PDX-1, PAX-6 and NKx2.2 gene were detected by semiquantity RT-PCR. Results: Compared with the control group, the PDX-1, PAX-6 and NKx2.2 gene expressions were significantly increased after co-cultured with GLP-1 for 1 day (P 〈 0.05). The effect was shown in a time-dependent manner. All three gene expressions reached the peak on the 5th day. Conclusion: GLP-1 can improve the function of pancreatic islet by regulating the gene expressions of transcription factors in β cells.Objective: To observe the effect of glucagon-like peptide 1 (GLP-1) on the gene expressions of transcription factors (PDX-1, PAX-6 and NKx2.2 ) in freshly isolated rat pancreatic islets and investigate the associated physiological and therapeutic implication of GLP-1. Methods: The isolated rat islets were incubated with 10 nmol/L GLP-1 for 1, 3 and 5 days, respectively. Total cellular RNA was extracted and the expressions of PDX-1, PAX-6 and NKx2.2 gene were detected by semiquantity RT-PCR. Results: Compared with the control group, the PDX-1, PAX-6 and NKx2.2 gene expressions were significantly increased after co-cultured with GLP-1 for 1 day (P 〈 0.05). The effect was shown in a time-dependent manner. All three gene expressions reached the peak on the 5th day. Conclusion: GLP-1 can improve the function of pancreatic islet by regulating the gene expressions of transcription factors in β cells.

关 键 词:glucagon-like peptide 1 ISLET transcription factor diabetes mellitus 

分 类 号:R587.1[医药卫生—内分泌]

 

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