人巨细胞病毒先天性潜伏感染再激活致老年小鼠肝脏的损伤  

Study on liver damage induced by reactivation of congenital latent infection of human cytomegalovirus in aged mice

作　　者：胡雪影[1] 王明丽[1] 

机构地区：[1]安徽医科大学微生物学教研室

出　　处：《世界华人消化杂志》2006年第12期1146-1150,共5页World Chinese Journal of Digestology

基　　金：教育部科学技术研究重点项目;No.01052;安徽省"十五"生物医药重大科技专项;No.01303003~~

摘　　要：目的:建立HCMV先天性潜伏感染再激活致肝脏损伤的BALB/c老年小鼠模型,初步探讨HCMV潜伏再激活感染的致病机制.方法:建立HCMV先天性感染小鼠的模型,将子代小鼠饲养于SPF级屏障系统中18mo后,从子代小鼠中分别取病毒感染组18只、细胞对照组9只,随机将病毒感染组中半数进行环磷酰胺激活,建立HCMV潜伏再激活感染鼠模型,按照实验设计分别处死细胞对照组、病毒潜伏组和病毒潜伏再激活组小鼠,无菌取出小鼠肝组织,进行病毒分离、PCR、RT-PCR及HE染色.结果:潜伏再激活组小鼠的肝组织匀浆上液中分离出HCMV,在病毒分离出现细胞病变效应(CPE)的细胞培养液中检测HCMVDNA;HE染色证实肝组织有炎性改变,肝细胞肿大,核内有嗜酸性包涵体等;PCR检测到HCMVDNA,RT-PCR检测到组织中HCMVmRNA.病毒潜伏组仅PCR检测到HCMVDNA,其它与细胞对照组一样均是阴性.结论:HCMV先天性潜伏感染再激活导致肝脏病理损伤.AIM： To establish the model of liver damage induced by reactivation of congenital latent infection of human cytomegalovirus （HCMV） in aged mice, and to explore the pathogenic mechanism of HCMV reactivation. METHODS： Firstly, the mouse model of congenital latent HCMV infection was established. After raised in specific pathogen free （SPF） barrier system for 18 mo, 9 offspring mice served as cell controls and 18 were taken out for experiment, half of which were treated with cyclophosphamide to reactivate the HCMV infection. The mice without HCMV infection were used as normal controls. All the mice were sacrificed to obtain liver tissues for virus isolation, polymerase chain reaction （PCR）, reverse transcription PCR （RT-PCR） and HE stainging. RESULTS： HCMV was isolated from the supernatant of liver tissue homogenate only in the group of infectionreactivated mice. HCMV DNA was detected by PCR in the supernatant of cell culture with cytopathogenic effect （CPE）. Pathological changes in liver consisted of swollen cytoplasm, destroyed nuclei of hepatocytes, distinct intranuclear inclusion in the hepatocytes, and predominant infiltration of phagocytic cells. HCMV DNA was found by PCR in the group of infection-reactivated and latently-infected mice, but HCMV mRNA was only found in the group of infection-reactivated mice by RT-PCR. CONCLUSION： Reactivation of congenital latent HCMV infection can induce liver damage in mice.

关 键 词：人巨细胞病毒 潜伏再激活感染 肝脏 婴儿肝炎综合症 

分 类 号：R575[医药卫生—消化系统]