急性髓细胞性白血病细胞培养上清对CD4^+和CD8^+ T细胞增殖和凋亡的影响  

Effects of Acute Myeloid Leukemia Cell Supernatant on the Proli-feration and Apoptosis of CD4^+ and CD8^+ T Cell Subsets

作　　者：王兴兵[1] 刘隽[2] 贺艳丽[2] 谷俊侠[2] 郑金娥[2] 姚军霞[2] 杨晶[2] 李小青[2] 黄士昂[2] 

机构地区：[1]安徽医科大学附属安徽省立医院血液科,合肥230001 [2]华中科技大学同济医学院附属协和医院血液病研究所干细胞研究与应用中心,武汉430022

出　　处：《中国实验血液学杂志》2006年第3期455-459,共5页Journal of Experimental Hematology

基　　金：国家杰出青年基金资助项目(编号30225038);科技部973项目(编号001CB510103)

摘　　要：为了研究不同的急性髓细胞性白血病(AML)细胞株培养上清对CD4+和CD8+T细胞增殖以及凋亡的影响,探讨AML的免疫抑制的形成机制,将3种AML细胞株(HL-60、NB4和U937)培养上清和普通培养液按不同比例混合,用于培养CFSE染色的淋巴细胞。抗CD3抗体和抗CD28抗体刺激3天后,标记7AAD和相应荧光抗体。利用流式细胞术检测不同T细胞亚群CFSE荧光强度和7AAD表达率的变化,分析其增殖和凋亡变化情况。结果表明:3种AML细胞株中有2种(HL-60和NB4)培养上清可显著抑制CD4+T细胞和CD8+T细胞的增殖,并随浓度增加而增强。同样,HL-60和NB4细胞株培养上清亦可抑制刺激后的CD4+T细胞的凋亡,但对刺激后的CD8+T细胞的凋亡并无明显影响。相反,HL-60和NB4细胞株培养上清可显著增加增殖的CD8+T细胞的凋亡。结论:AML细胞株培养上清液对CD4+T细胞和CD8+T细胞增殖的抑制以及对增殖的CD8+T细胞凋亡的诱导作用,可能是AML患者免疫功能缺陷的机制之一。To study the effects of supematant derived from acute myeloid leukemia （AML） cell lines on proliferation and apoptosis of CD4^＋ and CD8^＋ T cell subsets and to investigate the mechanism by which AML escapes from immune recognition, lymphocytes were labeled with CFSE and were stimulated with anti-CD3 and anti-CD28 in presence or absence of supematants from three AML cell lines （HL-60, NB4, U937 ）. After culture, cell suspensions were labeled with 7AAD and CD4 PE （or CD8 PE）. Cells were then detected by flow cytometry and their proliferation and apoptosis were analyzed. The results showed that supematants from two of three cell lines （HL-60 and NB4 ） inhibited the proliferation of CD4^＋ and CD8^＋T cells, and the degree of inhibition showed a dose-dependent way. Similarly, the apoptosis of stimulated CD4^＋ T cells was inhibited, but stimulated CD8^＋ T cells remained unaffected by supematant from HL-60 and NB4. In contrary, the apoptosis of proliferative CD8＋ T cells were increased significantly by HL-60 and NB4 supernatant. It is concluded that soluble factors derived from AML cell lines inhibit the proliferation of CD4^＋ and CD8^＋ T cells and induce the apoptosis of proliferative CD8^＋ T cells, that may be one of the mechanisms by which the immunity was suppressed.

关 键 词：急性髓细胞性白血病 CD4^+T细胞 CD8^+T细胞 细胞凋亡 细胞增殖 

分 类 号：R733.71[医药卫生—肿瘤] R730.3[医药卫生—临床医学]