大鼠脊髓背角内吗啡肽阳性终末与μ阿片受体阳性神经元的突触联系  

SYNAPTIC CONNECTIONS BETWEEN ENDOMORPHIN-IMMUNOREACTIVE TERMINALS AND μ OPIOID RECEPTOR-IMMUNOREACTIVE NEURONS IN THE SPINAL DORSAL HORN OF THE RAT

作　　者：吴利平[1] 陈涛[2] 张华[1] 黄晨[1] 李辉[2] 李云庆[2] 

机构地区：[1]第四军医大学西京医院惠宾病房 [2]第四军医大学解剖学教研室,西安710032

出　　处：《解剖学报》2006年第3期241-244,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金(30370458)资助项目

摘　　要：目的观察大鼠脊髓背角内吗啡肽阳性终末与μ阿片受体阳性神经元的突触联系。方法包埋前内吗啡肽免疫组织化学方法结合包埋前μ阿片受体免疫金颗粒标记的免疫电镜双标技术。结果内吗啡肽阳性终末以及μ阿片受体阳性胞体、纤维和终末主要分布于脊髓背角浅层(Ⅰ、Ⅱ层);在电镜下可见二氨基联苯胺(DAB)反应产物标记的内吗啡肽阳性终末与免疫金颗粒标记的μ阿片受体阳性神经元胞体及其树突之间形成以非对称性为主的突触联系,其中的轴-树突触明显多于轴-体突触。结论脊髓背角浅层内内吗啡肽与μ阿片受体阳性结构在分布方式上互相匹配,这种分布方式为其在外周痛信息传递的过程中发挥镇痛效应提供了形态学基础。Objective To investigate the synaptic connections between endomorphin-immunoreactive （ir） terminals and μ opioid receptor-ir neurons in the superficial layers （laminae Ⅰ and Ⅱ） of the rat spinal dorsal horn. Methods The double-labeling immunohistochemical electron microscopic technique, i. e. pre-embedding immunohistochemical detection of endomorphin combin with pre-embedding immuno-gold particles labeling technique to identify μ opioid receptor-immunoreaetivity, was used in the present study. Results μ opioid receptor-ir neuronal cell bodies, fibers as well as axon terminals were observed in laminae Ⅰ and Ⅱ of the spinal dorsal horn, meanwhile dense endomorphin-ir axon terminals were located mainly within the same region. Under electron microscope, the synaptic connections formed by endomorphin-ir axon terminals labeled with diaminobenzidine （DAB） reaction products and μ opioid receptor-ir neuronal cell bodies and dendritic profiles labeled with immuno-gold particles were observed. The principle synaptic type was asymmetric synapse. There were more axon-dendritic synapses than axon-somatic ones as encountered in the present study. Conclusion The distribution paμerns of endomorphin-ir and μ opioid receptor-ir structures match with each other in the superficial laminae of the spinal dorsal horn, that provides morphological base for the antinociceptive effects of endomorphin and μ opioid receptor in the processing of nociceptive information transmission.

关 键 词：内吗啡肽 Μ阿片受体 脊髓背角 镇痛 免疫电镜双标 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]