宫颈脱落细胞中cyclin E和Ki67的表达及意义  

Expressions of cyclin E and Ki67 in cervical exfoliated cells for screening cervical carcinoma

作　　者：刘丽华[1] 赵富玺[1] 郭俊成[1] 崔克[1] 米亚英[1] 

机构地区：[1]山西大同大学医学院微生物学教研室,山西大同037008

出　　处：《中华肿瘤防治杂志》2006年第9期666-668,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：山西省自然科学基金资助项目(20051115)

摘　　要：目的：探讨宫颈脱落细胞中cyclin E、Ki67的表达对宫颈癌高危人群筛查的意义。方法：采用免疫组化法对78例（不明意义的鳞状上皮非典型增生12例，宫颈上皮内瘤样痛变CINⅠ17例、CINⅡ～Ⅲ38例、浸润癌11例）宫颈脱落细胞标本进行cyclin E、Ki67检测。结果：cyclin E、Ki67在CINⅠ、CINⅡ～Ⅲ以及浸润癌标本中的阳性率均较ASCUS组高，差异有统计学意义，P〈0．005；CINⅠ组cyclin E的表达高于其他各组；Ki67的表达随着宫颈上皮细胞损伤程度加重而上升，浸润癌标本中阳性率最高；在CINⅠ组cyclin E和Ki672项指标特异度均为91.7％，灵敏度cyclin E（94．1％）高于Ki67（47．1％）。结论：采用宫颈脱落细胞检测cyclin E、Ki67作为辅助诊断指标应用于宫颈癌高危人群筛查，具有重要意义。OBJECTIVE： To evaluate the significance of cyclin E and Ki67 overexpressions in cervical exfoliated cells for screening patients at high risk of developing cervical carcinoma. MIETHODS： By the immuohistochemicalnalysis method, cyclin E and Ki67 in cervical exfoliated cells were delected in 78 cervical exfoliated epithelial samples, which were atypical squamous cells of undetermined significance （ASCUS , 12 cases）, cervical inter-aepithelial neoplasia（CIN） of type 1 （17 cases）, CIN2-3 （38 cases） and invasive carcinoma （11 cases） respectively. RESULTS： Cyclin E and Ki67 were all overexpressed in cervical preneoplasial and neoplasial cells, compared with those in ASCUS, P〈0. 005. The expression of Ki67 in cervical preneoplasial and neoplasial cells correlated well with cervical neoplasia, P〈0. 005. Cyclin E was overexpressed in CIN1 （94. 1%, P〈0.01）, and Ki67 was overexpressed in invasive carcinoma （90. 9 % ）. CONCLUSION ： The employment of cyclin E and Ki67 in combination for the primary screening of cervical cytology could not only reduce both the higher false-positive and false-negative rate of conventional Pap test, but increase its specificy and sensitivity.

关 键 词：宫颈肿瘤 子宫颈/细胞学 细胞周期蛋白E KI-67抗原 

分 类 号：R737.33[医药卫生—肿瘤]