家蝇乙酰胆碱酯酶基因的克隆与序列分析  

Cloning and sequence analysis of AChE gene from Housefly (Musca domestica)

作　　者：方晓东[1] 黄俊生[1] 

机构地区：[1]热带作物生物技术国家重点实验室,海南海口571101

出　　处：《中国热带医学》2006年第6期950-952,共3页China Tropical Medicine

基　　金：国家科技部"科技基础性工作和社会公益研究专项"(编号:2004DIB3J073);科技部"国家科技基础条件平台工作"(编号:2004DKA30560)资助

摘　　要：目的克隆家蝇Ace基因并进行表达,并对家蝇AChE序列进行分析,为利用基因工程进行酶改造提供必要的参考。方法结合已有的文献资料,利用生物信息学方法对家蝇AChE的序列进行分析,包括密码子偏爱性、系统发生、三维结构预测等。结果成功克隆家蝇Ace基因,并利用同源模建方法获得了家蝇AChE的三维结构,以果蝇AChE为参考,判断了家蝇AChE应属于AChE2家族。结论根据所得家蝇AChE的三维模型从理论上分析了因AChE中一个氨基酸突变导致家蝇产生有机磷抗性的可能原因,并为半理性改造家蝇乙酰胆碱酯酶提供了三维模型。Objective To clone acetylcholinesterase（AChE） gene from Housefly （Musca domestica ） in order to provide necessary reference for genetic engineering study, Methods The sequence of AchE was, by means of bioinformatics, analyzed ineluding eodon bias, systemic genesis, predicting of three - dimentional structure by using bioinformaties. Results AChE gene fi-om housefly was cloned and AChE three - dimentional structure was obtained by homologous modeling. ACHE, belonged to AChE 2 family, from fruitfly was taken as reference, Conclusion Based on housefly＇s AChE three - dimentional structure it is theoretically indicating the possibility that a mutation of an amino acid in Housefly＇ s AChE can lead to the resistance of housefly to organic phosphorus pesticide,

关 键 词：乙酰胆碱酯酶 序列分析 进化树 三维结构 同源模建 

分 类 号：R384.2[医药卫生—医学寄生虫学]