SARS CoV抗原决定簇的预测和制备  

Prediction and production of epitope of SARS-CoV

作　　者：王宣军[1] 吉海滨[1] 张秀霞[1] 李洋[2] 方勇[1] 王志武[1] 盛军[1] 

机构地区：[1]长春生物制品研究所生物技术研究室,长春130062 [2]吉林大学生命科学学院

出　　处：《中国公共卫生》2006年第6期704-705,共2页Chinese Journal of Public Health

摘　　要：目的获得严重急性呼吸道综合征(SARS)冠状病毒(CoV)中和性抗原蛋白。方法使用Blast工具将SARS S蛋白的氨基酸序列与其他蛋白进行比对分析。冠状病毒鼠肝炎病毒的刺突蛋白在S2段存在中和性抗体表位。通过对鼠肝炎病毒和SARS CoVS2的氨基酸序列分析,SARS CoV的S2段类似于鼠肝炎病毒的S2段。使用DNASTAR软件对2段的相似性进行分析。选取SARS CoVS2蛋白基因在大肠埃希菌中进行表达。表达产物进行免疫印迹分析。结果在SARS刺突蛋白C端发现了与其他冠状病毒S2蛋白具有较高的同源性的片段。在SARS病毒刺突蛋白的S2段可能存在中和性表位。将该肽段在大肠埃希菌中获得表达。表达的蛋白片段可以和来自SARS恢复期的人抗SARS-CoV免疫球蛋白特异性结合。结论表达的蛋白片段有可能成为预防SARS的候选疫苗。Objective To obtain the peptide of severe acute respiratory syndrome（SARS） - CoV contained the neutralizing B cell zpitope. Methods The amino acid sequence of major structure protein on the outer membrane of SARS- CoV was analyzed by blast with known protein, The coronvirus mouse hepatitis virus（ MHV）contained a neutralizing epitope in the S2region. The SARS - CoV contained the same region with MHV in the S2 segment. The amino acid sequence of the neutralizing epitope region of SARS - CoV and MHV was analyzed with the DNASTAR software. The gene of the peptide contain the selected region was expressed in E. coli. The expressed peptide was identified by Western - blotting. Results A conserved segment which named S2 segment of coronvirus was dig out in the C - terminal of the spike protein of SARS - CoV. The SARS- CoV may contained a neutralizing epitope in the $2 region. The gene contained the selected region was expressed in E. coll. The expressed peptide could be recognized by the human anti - SARS - CoV antibody coUected from the convalescence patients infected SARS. Conclusion The expressed peptide may be a selected vaccine for prevention of SARS.

关 键 词：表位预测 SARS-COV 表达 

分 类 号：R395.1[哲学宗教—心理学] R563.1[医药卫生—医学心理学]