水飞蓟素脂质体对小鼠CCl_4急性肝损伤保护作用的实验研究  

The protective effect of silymarin liposomes against experimental liver injury in mice

作　　者：许红霞[1] 高晓黎[1] 

机构地区：[1]新疆医科大学药学院药剂学教研室,新疆乌鲁木齐830054

出　　处：《新疆医科大学学报》2006年第5期421-423,共3页Journal of Xinjiang Medical University

摘　　要：目的：研究水飞蓟素脂质体（L-SIL）对四氯化碳（CCl4）所致小鼠急性肝损伤的保护作用.方法：昆明种小鼠80只,随机分为空白组、CCl4急性肝损伤模型组及L-SIL低、中、高剂量组和益肝灵（T-SIL）低、中、高剂量组共8组,测量并比较各组小鼠血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）水平及肝组织匀浆中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）水平和丙二醛（MDA）含量,并观察各组小鼠肝组织病理学改变.结果：与CCl4急性肝损伤模型组相比,L-SIL各剂量组血清ALT、AST水平明显降低（P〈0.05～0.01）,L-SIL各剂量肝组织匀浆GSH-PX水平及L-SIL高剂量组SOD水平明显升高（P〈0.05～0.01）,并具有剂量依赖性,L-SIL高剂量组肝组织匀浆MDA含量明显降低（P〈0.01）.L-SIL对ALT、SOD、GSH-PX水平的作用优于相应剂量T-SIL组（P〈0.05～0.01）.组织学观察L-SIL中、大剂量组的肝损伤明显轻于模型组和相应剂量T-SIL组.结论：L-SIL对小鼠CCl4肝损伤有明显的保护作用,且其保护作用明显优于T-SIL.Objective： To investigate the protective effect of silymarin liposomes（L-SIL） on the CCl4 in duced acute liver injury in mice. Methods： Kun ruing mice were randomly divided into normal control group, model control group, high, middle and low dose groups of L-SIL and high, middle and low dose groups of T-SIL. The model of hepatic damage were induced by CCl4. The Alanine aminotransferase （ALT）, Aspartate aminotransferase（AST） levels in serum , malondialdehyde（MDA） content, superoxide dismutase（SOD）, glutathione peroxidase（GSH-PX） levels in liver homogenate were examined and dimensions of hepatic damage in the histopathology were observed. Results： L-SIL could inhibit the rising of set um ALT, AST levels caused by CCl4 in dose-response manner （P 〈0.05-0.01）. Meanwhile, L-SIL also decreased MDA content （P 〈0. 01） and prevented the reduction of SOD, GSH-PX levels in the liver ho mogenate （P 〈0. 05-0.01）. The effects of L-SIL on the ALT level in serum and SOD, GSH-PX levels in liver homogenate against experimental liver injury in mice were more powerful than that of T-SIL of the same dose （P 〈0. 05-0.01）. The liver pathological observation showed that the liver injury of L-SIL （150,300 mg/kg. d） were significantly less than that of the model group and T- SIL of the same dose. Conclusion： The protective effect of L-SIL on the CCl4 induced acute liver injury in mice was more potent than that of T-SIL in the same dose.

关 键 词：水飞蓟素脂质体 益肝灵片 肝损伤 

分 类 号：Q949.783.5[生物学—植物学] R-332[医药卫生]