实验性变态反应性脑脊髓炎T淋巴细胞增殖和肿瘤坏死因子-α变化的意义(英文)  

Study on T cell proliferation and TNF-α abnormalities in Lewis rats with active experimental autoimmune encephalomyelitis

作　　者：吕佩源[1] 宋春风[2] 高昌星[3] 井上敦[3] 

机构地区：[1]河北省人民医院神经内科 [2]Laboratory of Electron Microscopy, Hebei Medical University, Shijiazhuang 050017, China [3]Department of Neurology, Shinshu University School of Medicine , Asahi 3-1- 1 , Matsumoto 390-8621, Japan

出　　处：《疑难病杂志》2006年第3期161-163,共3页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的探讨实验性变态反应性脑脊髓炎(EAE)T淋巴细胞增殖和肿瘤坏死因子(TNF)α变化的意义。方法从豚鼠(GP)脊髓中分离髓鞘碱性蛋白(MBP),主动免疫Lewis大鼠诱导EAE成功后,观察EAE组临床症状,并进行临床评分;应用淋巴细胞增殖试验测定EAE大鼠淋巴结T淋巴细胞增殖程度;应用ELISA技术测定血清TNFα水平。结果EAE临床发病过程中,T淋巴细胞增殖活跃,血清TNFα水平异常升高,并与临床症状呈正相关。结论EAE时T淋巴细胞增殖旺盛和TNFα生成增多,进而导致血脑屏障(BBB)的破坏,炎细胞浸润,从而诱发EAE。Objective To explore the relationship between T cell proliferation, serum tumor necrosis factor （TNF）-α abnormalities and clinical course of experimental autoimmune encephalomyelitis （EAE） in Lewis rats. Methods The rats of EAE were examined for the development of neurological signs of disease, T cell proliferation responses and TNF-α concentrations. Results There was an active T cell proliferation, and a marked TNF-α increase which paralleled with clinical onset in EAE. Conclusion Our studies suggest that T cell proliferation and increasing of TNF-α result in blood-brain barrier （BBB） breakdown and significant inflammatory cell recruitment which leads to EAE.

关 键 词：多发性硬化 脑脊髓炎 变态反应性 T淋巴细胞增殖 肿瘤坏死因子-Α 大鼠 

分 类 号：R744.53[医药卫生—神经病学与精神病学]