短杆菌属产胆固醇氧化酶的发酵条件优化  

Fermentation production of cholesterol oxidase by Brevibacterium sp.

作　　者：张锐[1] 许晓燕[1] 孟尧[1] 卢戌[1] 武翠玲[1] 姚明明[1] 孟延发[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,四川成都610064

出　　处：《中国酿造》2006年第6期23-28,共6页China Brewing

摘　　要：短杆菌属(Brevibacterium sp.)是胆固醇氧化酶高产菌,对其进行底物诱导及发酵条件的优化,其最适培养基为蔗糖0.3%,酵母膏0.2%,蛋白胨0.3%,牛肉膏0.3%,K2HPO4 0.1%,MgSO4 0.05%,pH6.8。最适培养条件为接种量5%,24℃培养20h,通气量为50mL培养基/250mL三角瓶,200r/min。结果表明,胆固醇氧化酶的酶活力可达到2439U/L,比未优化前195U/L提高了12倍。在pH6.5和温度54℃条件下测得酶米氏常数Km值为7.1×10-5 mol/L。Brevibactenum sterolicum can produce relative high content of cholesterol oxidase （COD）. The optimization of cultivation conditions was selected through fermentation tests. The optimal culture medium was： sucrose 3 g/L, yeast extract 2g/L, peptone 3 g/L, beef extract 3g/L, K2HPO4 1 g/L, MgSO4 0.5 g/L, pH 6.8. The optimal culture condition was inoculum 5%, aeration 50 mL culture/250 mL flask, 200 r/min, cultured 20 hours at 24℃. Under the optimized cultivation conditions, the enzyme activity reached 2439U/L, improve 14 times than un-optimized 195U/L. The enzyme was found that Michaelis constant Km is 7.1×10.5 mol/L when 6.5 as the optimum pH and 54℃ as the optimum temperature.

关 键 词：胆固醇氧化酶 短杆菌属 

分 类 号：Q814[生物学—生物工程]