LKB1表达下调对培养海马神经元突触mEPSC的影响  

Effect of LKB1 knockdown on the mEPSC of cultured hippocampal neurons

作　　者：杜鹏[1] 王伟[1] 谢敏杰[1] 喻志源[1] 徐运兰[1] 张强[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《卒中与神经疾病》2006年第3期131-133,共3页Stroke and Nervous Diseases

基　　金：国家自然基金重点项目(30230140;30400142);教育部新世纪优秀人才支持计划(NCET-04-0695)

摘　　要：目的 研究离体培养的海马神经元LKB1表达下调对于神经元微小兴奋性突触后电流（mEPSC）的影响。方法 选用17 d的胚胎大鼠培养海马神经元，分别用电穿孔的方法转染CAG-RE质粒和LKB1 RNAi质粒，培养10～12 d后进行电生理记录，选用全细胞膜片钳方式及自由记录模式，细胞外液加TTX阻断动作电位，加Bicucullin抑制GABA电流，记录神经元的mEPSC，比较2组神经元的mEPSC频率和幅度的差别。结果 转染CAG-RE的神经元mEPSC幅度平均为25．6 pA，频率平均为（5．21±0．25）Hz，是基线的99．8％；转染LKB1 RNAi的神经元mEPSC幅度平均为35．1 pA，频率平均为（5．79±0．27）Hz，是基线的127．1％；比较2组间频率、幅度变化，差别有显著性意义（P〈0．05）。结论 LKB1基因表达下调增强了培养海马神经元突触传递的效率。Objective To study the effect of LKB1 knockdown on the mEPSC of cultured hippoeampal neurons. Methods Used E17 cultured hippocampal neurons of SD rat, transfected neurons with CAG-RE and LKB1 RNAi. Whole-cell recording was used to observe the mEPSC of the 10div cultured neurons. Compared the amplitude and frequency of mEPSC between the neurons transfected with CACrRE and that of transfected with LKB1 RNAi. Results Compared with the neurons transfected with CAG-RE（control）, both amplitude and frequency of the neurons transfeeted with LKB1 RNAi enhanced. There was significant difference between the two kinds of neurons（P〈0.05）. Conclusions Knockdown of LKB1 enhanced the synapse activity in cultured hippocampal neurons.

关 键 词：LKB1 RNAI 海马神经元 MEPSC 突触 

分 类 号：R741[医药卫生—神经病学与精神病学]