CpG ODN对初生仔猪外周血CD4^+和CD8^+T淋巴细胞的影响  

Effect of CpG DNA on CD4^+ and CD8^+ T Lymphocyte Subpopulations in Blood of Newborn Piglets

作　　者：张玲华[1] 郭勇[1] 田兴山[2] 周风珍[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510640 [2]广东省农科院农业生物技术研究所,广东广州510640

出　　处：《华南理工大学学报（自然科学版）》2006年第5期63-67,共5页Journal of South China University of Technology(Natural Science Edition)

基　　金：广州市科技攻关重点项目(2003Z2-E0221);广东省农业攻关项目(2003B21403)

摘　　要：以猪伪狂犬病毒为模式病毒,研究了CpG ODN对仔猪外周血中CD4+,CD8+T淋巴细胞的影响.实验结果表明:疫苗组初生仔猪血液中CD4+/CD8+T淋巴细胞比例随年龄的增加逐渐降低,而CpG ODN能有效抑制其降低(P<0.05);CpG ODN的使用也可以阻止伪狂犬病毒导致的血液中CD4+T淋巴细胞比例的降低;联合免疫CpG ODN和猪伪狂犬病毒活疫苗,可以诱导初生仔猪血清中猪伪狂犬病毒特异性抗体迅速产生并达到较高的水平(P<0.05);这表明CpG ODN能显著增强动物的免疫应答能力.The effect of immunostimulatory CpG oligodeoxynucleotides （ CpG ODN） on CD4^＋ and CD8 ^＋T lymphocyte subpopulations in the blood of newborn piglets was investigated with or without pseudorabies attenuated （PSA） vaccine. Experimental results suggest that the CD4 ^＋/CD8 ^＋ ratio decreases with the age of newborn pigs inoculated with the vaccine only, but keeps steady in pigs treated with CpG ODN and PSA vaccine （ P 〉 0.05 ） , that CpG ODN can effectively prevent the decrease in the proportion of CD4 ^＋ T cell caused by PSA vaccine, and that high titer of PSA specific antibody can be tested in the serum of newborn piglets immunized by both PSA vaccine and CpG ODN （P 〈 0.05）. All these indicate that CpG ODN can greatly enhance the immune response of animals.

关 键 词：CPG T淋巴细胞 免疫应答 初生仔猪 免疫增强荆 伪狂犬病活疫苗 

分 类 号：S852.5[农业科学—基础兽医学]