赤子爱胜蚓全长cDNA文库的构建及初步鉴定  

Construction and primary identification of full-length cDNA library of Eisenia foetida

作　　者：许若丹[1] 钟理[1] 李继刚[1] 张伟[1] 柳伟强[1] 王志强[2] 吴策[1] 赵美玲[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]中国21世纪议程管理中心,北京100089

出　　处：《河北农业大学学报》2006年第3期79-82,共4页Journal of Hebei Agricultural University

摘　　要：以赤子爱胜蚓(Eisenia foetida)为材料,λTriplex2TM为载体,利用SMART技术构建全长cDNA文库,从而筛选全长目的基因并研究其结构和功能。经测定原始文库滴度为1.3×106pfu/mL,扩增总文库滴度为1.0×108pfu/mL,重组率达到99%以上,插入片段长度在1.1 kb左右。该文库的建立为功能性新基因的发现奠定了坚实的基础。To obtain a full - length cDNA library of Eisenia foetida for the purpose of gene screening and functioning study, total RNA extraction method from Eisenia foetida used is TRIZOL Reagent to construct a cDNA library by the carrier of λTriplex2^TM using the SMART cDNA Library Construction Kit. The quality of the constructed full - length cDNA library was evaluated. The primary library showed a titer of 1.3×10^6 pfu/mL of the cDNA insets, and the amplified library has a titer of 1.0 × 10^8. The recombinant rates were about 99 % and the average length of the cDNA inserts was about 1.1 kb. This constructed cDNA full - length library provided an essential resource for the further gene functional studies and gene discoveries of Eisenia foetida.

关 键 词：赤子爱胜蚓 全长CDNA CDNA文库 

分 类 号：Q785[生物学—分子生物学]