清热解毒类中药的生物鉴定法Ⅲ——穿心莲的生物鉴定  

USING BIOLOGICAL IDENTIFICATION Ⅲ TO TEST THE ANTI-BACTERIAL EFFECT OF ANDROGRAPHOLIDE

作　　者：王盛民[1] 张瑛[2] 赵子剑[2] 张恩户[2] 赵启鹏[2] 赫媛媛[2] 

机构地区：[1]西南交通大学药学院,四川峨眉山614202 [2]陕西中医学院,陕西咸阳712046

出　　处：《实用临床医药杂志》2006年第3期27-29,37,共4页Journal of Clinical Medicine in Practice

基　　金：四川省中医药管理局重点项目(2004A69);四川省乐山市重点项目(04SZ072)

摘　　要：目的 研究穿心莲的抑菌效应。探明穿心莲生物检定的适用剂量范围，建立标准曲线。方法 通过穿心莲对照药材对4种标准菌株的抑菌实验和穿心莲对敏感菌株的剂量效应曲线，构建适用于一点法的效应模式。结果 当穿心莲的浓度为0．11～0．55g／mL时，穿心莲的对数剂量和反应效应呈线性关系。结论 在上述浓度范围内，采用一点法，以穿心莲对照药材为标准品，对穿心莲药材和含穿心莲的中成药进行生物检定，方法可行。Objective This study aims at investigating the anti-bacteria effect of Andrographolide, showing the ranges of applicable dosage and establishing standard curves of Andrographolide. Methods An effective model applied to one-point method was well-established by measuring the effect of control medicinal materials of Andrographolide on inhibiting 4 standard bacterium strains and plotting the curves of dose-effect of Andrographolide in responseto sensitive bacterium strains. Results There was a good linear correlation between logarithm dosages and effect of Andrographolide when its concentrations ranged from 0.11 to 0.55 g/mL. Conclusion One-point method may be applicable to biological monitoring of Andrographolide or other Chinese medicines containing Andrographolide at the range of applicable concentrations above when control medicinal material of Andrographolide serves as standard sample.

关 键 词：穿心莲 标准菌株 脱水穿心莲内酯 HPLC 

分 类 号：R282.5[医药卫生—中药学]