氧诱导的视网膜病变小鼠模型建立方法的改良  

A Modified Mouse Model of Oxygen-induced Retinopathy

作　　者：丁小燕[1] 梁小玲[1] 谢素贞[1] 朱晓波[1] 唐仕波[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《眼科学报》2006年第2期98-102,共5页Eye Science

基　　金：广东省自然科学基金资助项目(05001786);国家自然科学基金资助项目(30471849)

摘　　要：目的:建立简单、稳定、成模率高的氧诱导的视网膜病变小鼠模型。方法:用改良法(A组)和传统方法(B组)分别建立氧诱导的视网膜病变的C57BL/6小鼠模型,比较两组的母鼠死亡率、乳鼠成活率和成模率,视网膜冰冻切片GSL染色免疫组化观察两组的视网膜新生血管,荧光素灌注视网膜铺片检测视网膜无灌注区和新生血管的面积。结果:改良法(A组)与传统法(B组)相比较,乳鼠成活率未降低,且具有母鼠死亡率低,成模率高,动物消耗量少的特点。改良法制作的小鼠模型视网膜无灌注区和新生血管面积较传统法多而典型。结论:改良法操作简单,能构建出高效、稳定、典型的视网膜新生血管模型。该方法值得推广和借鉴。Purpose： To set up a simple and effective oxygen-induced retinopathy （OIR） mouse model. Methods： Eyes from P17 mice in modified OIR group（Group A）and traditional OIR group （Group B） were snap frozen for immunohistochemical analysis with antibodies against GSL. Flat-mounted, fluorescein-conjugated dextran-perfused retinas were examined to assess the retinal vaseulature.The mortality of nursing mothers, survival rate of modeling mice and neovascularization ratio in Group A were obtained and compared with those in Group B. Results： Loss of the central retinal vasculature and the neovascular tufts were observed in GSL retinal immunohistochemical analysis. A vascular area and neovasculature area were significantly larger and more typical in Group A than those in Group B. A lower mortality of nursing mothers, higher survival rate and neovascularization ratio of modeling mice was achieved in Group A. Conclusions： This modified oxygen-induced retinopathy mouse model is a simple,effective method, with more typical neovasculature were obtained.

关 键 词：氧诱导的视网膜病变 动物模型 小鼠 

分 类 号：R-332[医药卫生]