早期糖尿病NOD小鼠视网膜HIF-1的表达  

The Expression of HIF-1 in the Early Diabetic NOD Mice

作　　者：李才锐[1,2] 胥延[3] 姜德咏[1,4] 洪卫[5] 郭小健[1] 王平[1] 李雯霖[1] 

机构地区：[1]中南大学湘雅二医院眼科 [2]大理医学院附属医院眼科,大理671000 [3]昆明医学院附属第二医院眼科 [4]湖南博雅眼科医院,长沙410007 [5]大理医学院附属医院眼科

出　　处：《眼科学报》2006年第2期107-111,共5页Eye Science

基　　金：湖南省科技厅基金资助项目(04SK3034/3)

摘　　要：目的:探讨糖尿病NOD小鼠视网膜HIF-1α的表达。方法:NOD小鼠随机分为正常对照组和糖尿病组,分别于糖尿病2、4、6、8和12周处死正常对照组和糖尿病组小鼠,摘除眼球后分离视网膜。Westernblot方法检测视网膜HIF-1α的表达,免疫组化方法检测视网膜上HIF-1α的表达。结果:与正常对照组相比,糖尿病组小鼠血糖明显增高,体重明显下降(P<0.01);Westernblot显示糖尿病6周时视网膜HIF-1α低水平表达,12周时HIF-1α表达增高;免疫组化显示糖尿病组12周时视网膜内层HIF-1α表达增加,而未见视网膜外层HIF-1α表达。结论:糖尿病早期视网膜HIF-1α表达增高,说明在糖尿病早期视网膜发生缺血缺氧;HIF-1α可能参与了糖尿病早期视网膜缺血缺氧的继发损害过程。Purpose： To study the retinal expression of HIF-1α in the diabetic NOD mice. Methods ： NOD mice were divided into normal control group and diabetes group randomly, sacrificed at 2,4,6,8 or 12 weeks. Eyes were enucleated and retinas were isolated and used for western blot, or eyes were sectioned for immunohistochemical staining for HIF-1α1. Results： Compared to the normal control group, blood sugar of diabetes mice increased significantly but the weights decreased significantly （P 〈 0.01 ）. Western blot of retinal lysates showed low levels of HIF-1α at the 6th weeks that were markedly increased at the 12th weeks in the diabetes group. Immunohistochemistry showed increased staining for HIF-1 in the inner retina, but not in the outer retina. Conclusion： The retinal HIF-1α levels increasing showed that retinal ischemia happening in the early diabetes. HIF-1α may participate in the secondary damage of retinal ischemia induced by early diabetes.

关 键 词：视网膜 低氧诱导因子-1Α 

分 类 号：R774.1[医药卫生—眼科]