冰醋酸固定液对视网膜组织固定效果的探讨  

The Investigation of FFA Fixative Solution for Retina

作　　者：张文忻[1] 李永平[1] 林健贤[1] 聂莉[1] 张卉颖[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《眼科学报》2006年第2期112-114,共3页Eye Science

基　　金：广东省科技计划基金资助项目(2003A3020302)

摘　　要：目的:寻找对视网膜固定效果好的固定液。方法:用福尔马林、酒精、冰醋酸混合(glacialaceticacid,FAA)固定液和10%中性甲醛固定液分别对视网膜组织固定,作HE染色和免疫组化染色,镜下观察其差异。结果:FAA固定液的标本视网膜结构清晰,细胞排列紧密,脱片程度低,适用于视网膜组织的固定。结论:FAA固定液固定视网膜组织的效果优于10%中性甲醛固定液。Purpose： To search for an effective fixative solution for retina. Methods： One group of retinas were fixed in an admixture of formalin, dehydrated alcholhol and glacial acetic acid （FFA）.The other group of retinas were fixed in 10% neutral formalin.HE and immunohisto chemistry were performed.Then compare the difference under the light microscope. Results： The structure of the retinas fixed in FFA fixative solution was clear.The cells were arranged and compacted.Few exfoliation of the tissue from the glass slide was observed. Conclusion： FFA fixative solution is superior to 10% neutral formalin for the fixation of retina.

关 键 词：视网膜 冰醋酸 固定液 

分 类 号：R774.1[医药卫生—眼科]