甘氨酸受体在大鼠心肌细胞的表达  

Expression of glycine receptor in rat cardiomyocytes

作　　者：张俊艳[1] 陆大祥[1] 戚仁斌[1] 王华东[1] 王彦平[1] 付咏梅[1] 陈梦飞[1] 赵雪[1] 李楚杰[1] 

机构地区：[1]暨南大学医学院病理生理教研室,广东广州510632

出　　处：《中国病理生理杂志》2006年第6期1070-1073,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助(No.30470718);广东省自然科学基金重点项目资助(No.04105844);广州市科技局重点项目资助(No.2004Z3-E4081)

摘　　要：目的:检测成年大鼠心肌组织和新生大鼠心肌细胞是否有甘氨酸受体(GlyR)mRNA和蛋白的表达。方法:选用成年雄性SD大鼠心肌组织和新生SD大鼠培养心肌细胞,提取总RNA和膜蛋白,用逆转录-巢式聚合酶链反应(RT-PCR)和蛋白免疫印迹(Western blotting)检测GlyRα1和β亚基的mRNA和蛋白表达。结果:成年大鼠心肌组织和新生大鼠培养心肌细胞均有与脊髓组织GlyRβ亚基极为相似的mRNA和蛋白的表达;对于GlyRα1亚基,仅在新生SD大鼠培养心肌细胞发现有mRNA的表达。结论:本研究证实在成年大鼠心肌组织和新生大鼠心肌细胞中有与脊髓组织相似的GlyR亚基的表达,提示大鼠心肌细胞的膜上有GlyR的存在。AIM： To study the expression of glycine receptor （GlyR） in adult rat cardiac tissue and cultured cardiomyocytes from neonatal rat hearts. METHODS： Total RNA and membrane protein were extracted from cardiac tissue of adult rats and primary cultured cardiomyocytes from neonatal rat hearts, α1 and β subunits mRNA and protein of GlyR were detected with the method of reverse transcription nest DNA polymerase chain reaction （RT- PCR） and Western blotting. RESULTS： The mRNA and protein expression of β subunit were identified in the adult rat heart tissue and cultured cardiomyocytes,similar to the sequence and immunngenicity generated from GlyR β subunit of the rat spinal cord; for αl subunit, mRNA was found in only cultured cardiomyocytes. CONCLUSION： These data demonstrate that adult rat cardiac tissue and cultured cardiomyocytes from neonatal rat hearts express mRNA and protein of GlyR subunits similar to the GlyR that expresses in the spinal cord, suggesting that GlyR exists in the membrane of the rat cardiomyocytes.

关 键 词：受体 甘氨酸 印迹法 蛋白质 大鼠 心肌细胞 

分 类 号：R363[医药卫生—病理学]