益骨胶囊含药血清在共育体系中对大鼠成骨细胞增殖、ALP活性及IL-11 mRNA表达的影响  

Effect of yigu capsule drug-containing serum on proliferation, ALP activity and expression of interleukin-11 mRNA in rat osteoblasts in the co-culture

作　　者：傅淑平[1] 张荣华[1] 徐立群[1] 

机构地区：[1]暨南大学药学院中药学教研室,广东广州510632

出　　处：《中国病理生理杂志》2006年第6期1171-1173,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金项目资助(No.30472274);广东省自然科学基金重点项目资助(No.04105837);广东省社会发展计划资助重点引导项目(No.2004B33001004)

摘　　要：目的:观察益骨胶囊含药血清在成骨-破骨细胞共育体系中对SD大鼠成骨细胞(OB)增殖、碱性磷酸酶(ALP)活性及白细胞介素-11(IL-11)mRNA表达的影响。方法:(1)取1d龄SD大鼠颅骨分离培养OB,取5d龄SD大鼠四肢股骨、胫骨分离培养破骨细胞(OC),建立细胞上清相通但细胞间不相互混杂的平面式成骨-破骨细胞共育体系,实验分为两组(含药血清组和对照组)。(2)MTT法检测OB增殖,氨基安替吡啉测酚法测定ALP活性,FQ-PCR法测定IL-11mRNA相对表达量。结果:含药血清组中OB的A值、ALP活性、IL-11/β-actin比值均高于对照组(P<0·05)。结论:益骨胶囊含药血清在共育体系中可促进OB增殖、增强OB ALP活性,提高IL-11mRNA的表达。AIM： To investigate the effect of yigu capsule drug - containing serum on proliferation, ALP activity and expression of interleukin - 11 mRNA in Sprague - Dawley rat osteobalsts in the co - culture system. METHODS： （ 1 ） Osteoblasts and osteoclasts were isolated from 1 and 5 - day - old Sprague - Dawley rats, respectively. The osteoblasts - osteoclasts co - culture system was built to prevent the two kinds of cells from contact and allow the media to exchange. The experiment included two groups, drug- containing sera group and control group. （2） The osteoblasts proliferation, AlP activity and expression of interleukin - 11 mRNA were detected by the MTT, 4- aminoantipyrine spectrometric methods and FQ- PCR, respectivdy. RESULTS： In drug - containing sera group, the osteoblasts proliferation, ALP activity, expression of IL - 11 mRNA were higher than those of control group （ P 〈 0.05）. CONCLUSION： Rat sera containing yigu capsule can obviously enhance the osteoblast proliferation, ALP activity and expression of IL-11 mRNA.

关 键 词：益骨胶囊 成骨细胞 白细胞介素11 碱性磷酸酶 MRNA表达 

分 类 号：R363[医药卫生—病理学]