乳腺癌前哨淋巴结不同转移状态下局部淋巴结成熟树突状细胞的研究  

DC-LAMP^+ dendritic cells in sentinel and non-sentinel lymph nodes of breast cancer patients with different lymph node metastatic status

作　　者：黎剑[1] 詹文华[1] 黄美近[1] 蔡世荣[1] Andreas Bembenek Peter M.Schlag 

机构地区：[1]中山大学附属第一医院胃肠胰外科,广东广州510080 [2]CHARITE医科大学附属肿瘤外科医院,德国柏林d13125

出　　处：《中国病理生理杂志》2006年第6期1196-1198,共3页Chinese Journal of Pathophysiology

基　　金：柏林癌症协会ErnestvonLeyden奖学金资助

摘　　要：目的:研究乳腺癌前哨淋巴结不同转移状态下前哨淋巴结与非前哨淋巴结成熟树突状细胞密度的改变。方法:回顾性分析79例符合研究标准的女性乳腺癌患者。根据前哨淋巴结的转移状况分为3组:A组(28例),所有淋巴结转移阴性;B组(25例),仅微小肿瘤出现于前哨淋巴结,包括B1组(16例),仅游离肿瘤细胞出现于前哨淋巴结;B2组(9例),仅微转移出现于前哨淋巴结;C组(26例),转移出现于前哨淋巴结,非前哨淋巴结有或无转移。所有前哨淋巴结及非前哨淋巴结蜡块切片均行与DC-LAMP抗体免疫反应的免疫组织学检查以确认成熟树突状细胞。蔡司图像分析系统定量分析每个淋巴结DC-LAMP阳性细胞的相对密度(DC-LAMP阳性细胞面积/淋巴结面积)。Wicoxon检验和Mann-Whitney检验分别用于DC-LAMP阳性细胞的相对密度的组内和组间比较。结果:DC-LAMP阳性细胞密度的组内比较显示A组和B组前哨淋巴结DC-LAMP阳性细胞平均密度较非前哨淋巴结高(P<0·05,P<0·01);C组前哨淋巴结DC-LAMP阳性细胞平均密度与非前哨淋巴结比较无明显差异(P>0·05)。组间比较显示各组前哨淋巴结DC-LAMP阳性细胞平均密度无显著差异;而B组(尤其B2组)非前哨淋巴结DC-LAMP阳性细胞密度较A组和C组显著升高(P<0·05,P<0·01)。结论:前哨淋巴结和非前哨淋巴结DC-LAMP阳性细胞平均密度在淋巴结肿瘤转移形成过程发生改变,揭示前哨淋巴结在肿瘤与引流淋巴结间相互作用中起重要作用。AIM： To invesfigate the density of mature dendritic cells （ DC） in sentinel lymph nodes （SLN） andnon- SLN in patients with ditiexent levels of metastatic involvement. METHODS： The female breast cancer patients were divided into 3 groups： group A, all lymph nodes without evidence for tumor cells; group B, small tumor deposits in SLN defined as： B1, isolated tumor cells in SLN, non- SLN without evidence of tumor cells; 132, micrometastases in SLN, non- SLN without evidence of tumor cells; gnmp C, in SLN, involved or un- involved non- SLN one section of all SLN and non- SLN of these paexamination by using the DC- LAMP- antibody for identification of mature dendritic cells. The density of the DC - LAMP^＋ - cells of each slide was quantified by a Zeiss imaging system enregistering the percentage of the area occupied by DC - LAMP^＋ - cells. The Wicoxon - test and Mann - Whitney- test were used to compare the mean density of DC - LAMP^＋ - cells of SLN and non - SLN individually in each group and interindividuaily between the differem gnmps, respectively. RESULTS： The DC - LAMP^＋ - cell density increased significantly in the SLN compared to non - SIN patients with tumor free lymph nodes and patients with micrometastases in the SLN （ P 〈0.05, P 〈 0.01）. No significant increase in DC- LAMP^＋ - cell density between the SLN of the three groups, but a significantly reduced DC - density in non - SLN of patients with micrometas- rases and isolated tumor cells was observed, compared to the other two groups （ P 〈 0.05, P 〈 0.01 ）. CONCLUSION： The resuits further underlined the prominent role of the SLN for tumor- lymph node interactions, revealing significant differences between SLN and non - SLN in regard to the density of DC - LAMP^＋ - cells.

关 键 词：乳腺肿瘤 前哨淋巴结 树突细胞 肿瘤转移 

分 类 号：R363[医药卫生—病理学]