微波消融肝癌对小鼠细胞免疫的影响  

Effect of microwave ablation of liver cancer on cellular immunity in mice

作　　者：谭进富[1] 吕明德[1] 刘大全[1] 王竹[1] 周忠信[1] 黄洁夫[1] 

机构地区：[1]中山大学附属第一医院肝胆外科,广东广州510080

出　　处：《中国病理生理杂志》2006年第6期1214-1217,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30471686)

摘　　要：目的:探讨不同温度微波消融肝癌对小鼠细胞免疫的影响。方法:建立C57BL/6J小鼠Hepa1-6肝癌皮下移植模型,分别采用45℃、50℃、55℃和60℃×180s的消融条件对肝癌进行微波消融。用流式细胞仪分析外周血CD4+、CD8+T细胞和NK细胞的比例,用LDH法测脾NK细胞和灭活的Hepa1-6细胞活化的脾细胞毒T淋巴细胞(CTL)的细胞毒活性。结果:50℃和55℃组消融后21d外周血CD4+、CD8+T细胞和NK细胞比例明显升高,60℃组消融后21d外周血NK比例明显升高,但消融后42d与对照组相比均无明显差异。50℃和55℃组消融后21d和42d脾CTL和NK细胞毒活性均明显增强,且均明显强于同期45℃组。50℃和55℃组消融后21d脾CTL细胞毒活性明显强于同期60℃组。结论:在一定消融温度条件下,肝癌微波消融能促进机体产生细胞免疫反应。AIM： To investigate the effect of microwave ablation of liver cancer on the cellular immunity in mice. METHODS： A C57BL/6J mouse model of liver cancer was established by subcutaneous injection of Hepa 1 - 6 cells. The tumors were subjected to microwave ablation under the ablation condition of 45 ℃, 50℃, 55℃ or 60℃ for 180 s. The CD4^＋ T cells, CD8^＋ T cells and natural killer cells （NK） in peripheral blood were detected by FACS. Tne cytotoxicity of splenic NK and splenic cytotoxic T lymphocytes （CTL） activated by inactivated Hepa 1 - 6 cells was assayed by LDH method. RESULTS： The proportions of CD4^＋ T cells, CD8^＋ T cells and NK cells in peripheral blood in 50℃ and 55℃ group at 21 d after ablation were signiticanfly and that of NK cells in 60℃ group was significantly increased. There was no significant difference between those in group 42 d after ablation and control. The cytotoxicities of splenic CTL and NK cells in 50 ℃ and 55 ℃ groups at 21 d or 42 d after ablation were significantly increased, and they were much higher than those in 45℃ gnmp at the same time. The cytotoxicities of splenic CTL in 50℃ and 55℃ groups at 21 d after ablation were much higher than that in 60℃ group at the same time. CONCLUSlON： Under a certain ablation temperature, microwave ablation of liver cancer promotes the cellular immunity.

关 键 词：消融 肝肿瘤 T淋巴细胞 杀伤细胞 天然 免疫 细胞 

分 类 号：R736[医药卫生—肿瘤]