吗啡依赖和复吸时大鼠脑内神经甾体水平的变化  

Effect of morphine dependence and relapse on the levels of neurosteroids in male rat brain regions

作　　者：王娜[1] 吴红海[1] 侯艳宁[1] 

机构地区：[1]白求恩国际和平医院药学部,河北石家庄050082

出　　处：《中国药理学通报》2006年第6期727-730,共4页Chinese Pharmacological Bulletin

基　　金：河北省自然科学基金资助项目(NoC2005000834)

摘　　要：目的探讨吗啡精神依赖和复吸对大鼠脑内神经甾体水平的影响。方法采用条件性位置偏爱(CPP)实验,吗啡诱导大鼠CPP形成,足底电击应激诱发CPP重建,高效液相色谱-质谱法测定大鼠额叶皮质、海马及血浆中孕烯醇酮(PREG)及其硫酸酯(PREGS)、别孕烯醇酮(AP)、脱氢表雄酮(DHEA)及其硫酸酯(DHEAS)的含量。结果5 mg.kg-1吗啡训练10 d诱导大鼠产生了稳定的CPP,间歇足底电击有效诱发CPP的重建。与对照组比较,吗啡CPP形成时,大鼠额叶皮质内DHEA、PREG水平升高(P<0.05),海马内DHEA水平降低(P<0.05);与吗啡消退组比较,足底电击诱发CPP重现时,大鼠额叶皮质内PREG水平降低(P<0.01),而海马内PREG水平升高(P<0.05)。结论大鼠额叶皮质和海马内神经甾体水平的变化可能与吗啡依赖和复吸有关。Aim To investigate the effect of morphine dependence and relapse on the levels of neurosteroids in male rat frontal cortex （Fc） and hippocampus （ Hc ）. Methods Morphine-induced conditioned place preference （CPP） paradigm in rat was used and footshock stress was given to rats to reinstate morphine CPP. The contents of pregnenolone （ PREG ） , pregnenolone sulfate （PREGS）, allopregnanolone （AP）, dehydroepiandrosterone （DHEA）, and dehydroepiandrosterone sulfate （DHEAS） in rat Fc homogenates, He homogenates and plasma were determined with liquid chromatography-mass spectrometry. Results 5 mg . kg^-1 morphine conditioning during 10 days resultedin acquisition of CPP and intermittent footshock reactivated morphine CPP following 7 days drug-free periods. Compared with saline group, morphine-induced CPP elevated DHEA and PREG contents in Fc （P 〈 0.05）, while decreased DHEA level in Hc （P 〈 0. 05）; Compared with morphine extinct group, footshock-reinstated morphine CPP decreased PREG content in Fc（ P 〈 0.01 ）, but elevated PREG content in Hc （P 〈 0. 05 ）. Conclusion The changes of neurosteroids in rat Fc and He may be related to the formation and relapse of morphine dependence.

关 键 词：吗啡依赖 复吸 神经甾体 条件性位置偏爱 脑 

分 类 号：R-332[医药卫生] R322.81