染料木素对离体豚鼠左室乳头肌收缩功能的影响  

Effects of genistein on contractility of isolated left ventricular papillary muscles in guinea pig

作　　者：吴金霞[1] 李红芳[1] 田治峰[2] 邱小青[2] 顾静[1] 贾忠健[3] 

机构地区：[1]兰州大学基础医学院生理学教研室 [2]兰州大学基础医学院医学机能实验室 [3]兰州大学化工学院天然有机室,甘肃兰州730000

出　　处：《中国药理学通报》2006年第6期743-746,共4页Chinese Pharmacological Bulletin

基　　金：中国博士后科学基金资助项目(No2003034442)

摘　　要：目的观察染料木素对离体豚鼠左室乳头肌收缩功能的影响,并探讨其作用机制。方法将离体豚鼠乳头肌置于装有KH液的灌流肌槽中,待平衡后,加入各种药物观察乳头肌收缩活动的变化。结果染料木素和异丙肾上腺素相似,可增强豚鼠左室乳头肌的收缩活动,染料木素(1、100μmol.L-1)的作用还具有明显的剂量依赖性。心得安(1μmol.L-1)和异搏定(0.5μmol.L-1)虽可明显阻断异丙肾上腺素(2μmol.L-1)的正性肌力作用,但对染料木素(100μmol.L-1)的心肌收缩增强效应无明显改变;同时发现染料木素(1、10μmol.L-1)对细胞外液Ca2+浓度升高而诱发的心肌收缩力增强也无明显影响。另外,它莫西芬(1μmol.L-1)可明显减弱染料木素的正性肌力作用,但正矾酸钠(1μmol.L-1)对其作用无明显影响。结论染料木素可增强心肌收缩活动,其作用与心肌细胞膜上的β肾上腺素能受体和钙通道激活以及酪氨酸激酶途径无关,可能与肌质网Ca2+的摄取和利用有关。Aim To study the effect of genistein on contractility of isolated papillary muscles in guinea pig and its mechanisms. Methods Isolated guinea pig papillary muscles were suspended in organ baths containing KH solution, and the effect of genistein on contractility of myocardium was observed after an equilibration period. Results Genistein and isoprenaline hydrochloride showed positive inotropic effects on contractility of myocardium in a dose-dependent manner（1-100 μmol · L^-1 ） Propranolol （1 μmol · L^-1 ） and verapamil hydrochloride （0. 5 μmol · L^-1 ） attenuated the positive inotropic effect of isoprenaline hydrochloride （2 μmol · L^-1） , but did not change the effect of genistein （100 μmol · L^-1 ）. Furthermore, the enhancement of the contractility induced by elevation of extracellular Ca^2＋ concentration in papillary muscles did not show any change after pretreatment with genistein （1, 10 μmol · L^-1）. Finally, the positive inotropic effect of genistein was attenuated partially by tamoxifen （1 μmol · L^-1）, however, was not influenced by sodium orthovanadate （1 μmol · L^-1）.Conclusions Genistein has the positive inotropic effect on guinea pig papillary muscles, which is not related to the activation of Ca^2＋ channel on cell membrane, β adrenoceptor and tyrosine kinase pathway,but this effect may involve in uptake and utilization of sarcoplasmic reticulum Ca^2＋.

关 键 词：染料木素 收缩活动 乳头肌 

分 类 号：R-332[医药卫生] R282.71