Fura-2荧光测定胞质Ca^(2+)实验中注意Cd^(2+)的荧光干扰作用  

The interference of Cd^(2+) with Fura-2 fluorescence in measurement of free cytoplasmic Ca^(2+)

作　　者：张政[1] 蔡炳祥[1] 丘钦英[1] 周家国[1] 关永源[1] 

机构地区：[1]中山大学中山医学院药理学教研室,广东广州510080

出　　处：《中国药理学通报》2006年第6期763-764,共2页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题(No30271503)

摘　　要：目的探讨Cd2+在Fura-2荧光测定胞质Ca2+过程中对荧光的干扰作用。方法培养的PC12细胞,在无钙溶液中利用Fura-2荧光探针测定胞质内Ca2+浓度。结果在thapsigargin诱导Fura-2荧光比值(F340/F380)上升后,CdC l2可以导致F340/F380进一步升高。将细胞膜破坏,Fura-2从胞质内溢出,CdC l2依然可以导致荧光比值明显上升。在无PC12细胞,无Ca2+的溶液中,加入Fura-2/AM,100μmol.L-1CdC l2可以导致F340/F380明显升高。结论Cd2+对Fura-2及Fura-2/AM都存在荧光干扰现象,使荧光比值增加。Aim To investigate the blockade of cadmium on store operated calcium channels and the fluorescence interference of cadmium with Fura-2. Methods PC12 ceils were used to determine the intracellular calcium concentration [ Ca^2＋ ] i indicated by change in Fura-2 fluorescence ratio（ F340/F380 ）. Resuits Introduction of cadmium induced a significant increase in Fura-2 fluorescence ratio following thapsigargin-evoked calcium entry; Besides, cadmium gave rise to a remarkable elevation in Fura-2 fluorescence ratio following breakdown of the plasma membrane by triton, a detergent. The Fura-2 fluorescence was increased in the presence of cadmium and Fura-2/AM, simultaneously in the absence of PC12 cells. Conclusion Cadmium can interfere with the Fura-2 fluorescence.

关 键 词：CD^2+ FURA-2 荧光增敏 PC12 

分 类 号：R392.2[医药卫生—免疫学] R348.2[医药卫生—基础医学]