建立脑内灌流6-OHDA所致大鼠纹状体细胞外液羟自由基升高的模型  

Induction of extracellular hydroxyl radical in striatum of rats by intracerebral perfusion of 6-OHDA

作　　者：王丹巧[1] 王巍[1] 赵德忠[1] 

机构地区：[1]中国中医研究院西苑医院老年病研究所,北京100091

出　　处：《中国药理学通报》2006年第6期765-768,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题(No30371824)

摘　　要：目的建立脑内灌流6-羟基多巴胺(6-OHDA)所致大鼠纹状体细胞外液羟自由基升高的模型,为老年退行性病变和其它氧化应激所致脑细胞损伤的研究和药物筛选提供可利用的方法。方法微透析脑内灌流6-OHDA造模,采用水杨酸捕获羟自由基,高效液相-电化学检测技术,对活体脑内羟自由基所形成的2,3二羟基苯甲酸(2,3-DHBA)和2,5二羟基苯甲酸(2,5-DHBA)进行测定。结果6-OHDA脑内灌流后,模型组大鼠纹状体细胞外液2,3-DHBA和2,5-DH-BA在75 m in分别为对照组的6.6和3.4倍;2,3-DHBA在观察的全程中,一直高于对照组(P<0.01);2,5DHBA大部分时间点也高于对照组(P<0.05或P<0.01);维生素EC组的2,3-DHBA有4个时点低于模型组(P<0.05或P<0.01),2,5-DHBA各时点均低于模型组,但差异无显著性。结论6-OHDA脑内灌流可以造成大鼠纹状体细胞外液羟自由基升高的急性模型。Aim To provide an approach to drug filtration and the research into damage of brain cell caused by senile retrograde affection and oxidative stresses, a model of increased extracellular hydroxyl radical in striatum of rats was established by intracerebral perfusion of 6-hydroxy dopamine （ 6-OHDA ）. Method Microdialysis was used to establish the model, hydroxyl radicals captured by salicylic acid yielded 2,3-dihydroxy benzyl acid （2,37DHBA） and 2,5-dihydroxy benzyl acid （2,5- DHBA） which were measured by HPLC-ED. Results After 75 minuets of the perfusion with 6-OHDA in brain of rats, the levels of 2,3-DHBA and 2,5-DHBA in the model group increased to 6.6 and 3.4 times compered with the control group respectively; and the 2,3-DHBA was still higher than that of the control group in the whole course of observation （P 〈 0. 01 ）, while 2,5- DHBA was higher than that of the control group among all time points （ P 〈0. 05 or P 〈0.01）. Conclusion Intracerebral perfusion of 6-OHDA. Can be used to establish a model of rising extracellular hydroxyl radical in striatum of rats.

关 键 词：脑内微透析 羟自由基 6-羟基多巴胺 水杨酸捕获 

分 类 号：R-332[医药卫生] R322.81